His標(biāo)簽，也稱為多組氨酸標(biāo)簽（polyhistidine），His6標(biāo)簽或hexa組氨酸標(biāo)簽，這是一種在轉(zhuǎn)染的細(xì)胞中目標(biāo)蛋白的C端或N端上由至少6個組氨酸殘基鏈接上所組成的氨基酸序列。

His-Tag是目前用于純化的融合標(biāo)簽中應(yīng)用最廣泛的特點：

a：標(biāo)簽較短可置于蛋白的N端或者C端

b：對蛋白的功能及下游應(yīng)用影響小

c：免疫原性低，可以和多種標(biāo)簽連用

d：純化方式非常簡單方便

這些屬性使His標(biāo)簽成為重組蛋白生產(chǎn)和分離的流行標(biāo)簽。然而，對于未純化的樣本，His標(biāo)簽蛋白的準(zhǔn)確定量樣品仍然很困難。

作為專業(yè)的生命科學(xué)醫(yī)藥原料供應(yīng)商，Cell Biolabs中國區(qū)金牌代理，艾美捷科技為您推薦：His-Tag 蛋白ELISA 試劑盒，這是一種基于競爭性ELISA測定法，專為快速檢測和定量His標(biāo)簽蛋白樣品（C 端或 N 端）而開發(fā)。

引用文獻(xiàn)：

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