脂質(zhì)立方相（LCP）結(jié)晶已經(jīng)發(fā)展成為膜蛋白結(jié)晶的一種重要方法，其中脂質(zhì)單油酸甘油酯（Monoolein）是創(chuàng)建穩(wěn)定LCP的首選。然而，在過去幾年中，短鏈脂質(zhì)7.7 MAG^[4,7]、7.8 MAG^[6-8]和7.9 MAG^[5,7]變得越來越受歡迎。最近在將高黏度脂質(zhì)立方相應(yīng)用于連續(xù)飛秒晶體學(xué)（LCP-SFX）方面的進(jìn)展^[1-3]，通過以下方式進(jìn)一步加速了LCP方法的成功：

• 將載有晶體的黏性LCP直接送入X射線自由電子激光（XFEL）光束中（與液體注射器相比，減少了樣品消耗)；

• 利用在LCP中生長(zhǎng)的固有小晶體；

• 避免了從LCP中進(jìn)行棘手的晶體裝載。

JBScreen LCP旨在高效篩選LCP中的結(jié)晶條件。上述表格中的每種LCP脂質(zhì)都能創(chuàng)建一個(gè)穩(wěn)定的脂質(zhì)立方相，可用于與JBScreen LCP進(jìn)行結(jié)晶設(shè)置。其96種條件是通過對(duì)192種成功通過in meso方法結(jié)晶并產(chǎn)生結(jié)構(gòu)的整合膜蛋白進(jìn)行數(shù)據(jù)挖掘得出的^[7]。該篩選按沉淀劑的濃度和類型排序，并且不含乙酸鎘。

文獻(xiàn)：

[1] Nogly et al. (2016) Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nat. Commun. 7:12314.

[2] Zhu et al. (2016) Serial Femtosecond Crystallography of Membrane Proteins. Adv. Exp. Med. Biol. 922:151.

[3] Batyuk et al. (2016) Native phasing of x-ray free-electron laser data for a G protein-coupled receptor. Sci. Adv. 2:e1600292.

[4] Coincon et al. (2016) Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters. Nat. Struct. Mol. Biol. 23:248.

[5] Li et al. (2015) Ternary structure reveals mechanism of a membrane diacylglycerol kinase. Nat. Commun. 6:10140.

[6] Fowler et al. (2015) Gating Topology of the Proton-Coupled Oligopeptide Symporters. Structure 23:290.

[7] Caffrey (2015) A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Acta Cryst F 71:3.

[8] Li et al. (2013) Crystal structure of the integral membrane diacylglycerol kinase. Nature 497:521.

[9] Misquitta et al. (2004) Rational design of lipid for membrane protein crystallization. Journal of Structural Biology 148:169.