面對(duì)復(fù)雜多變細(xì)胞模型，您是否為如何精確測(cè)量細(xì)胞內(nèi)的缺氧及氧化應(yīng)激水平而困擾？ 

是否為一組檢測(cè)而需購(gòu)買2套試劑盒的高昂成本而煩惱？

是否對(duì)傳統(tǒng)的檢測(cè)方法繁瑣且結(jié)果可靠性存疑？   

在生命科學(xué)的前沿探索中，準(zhǔn)確評(píng)估細(xì)胞的缺氧與氧化應(yīng)激狀態(tài)，對(duì)于揭示疾病機(jī)理、優(yōu)化治療策略至關(guān)重要。如今，Enzo Life Sciences傾情推出ROS-ID Hypoxia/Oxidative Stress Detection Kit（ENZ-51042-0125，ENZ-51042-K500），這是一款專為科研人員設(shè)計(jì)，二合一的創(chuàng)新工具，艾美捷為您推薦的ROS-ID 試劑盒直擊這一系列挑戰(zhàn)，以其獨(dú)特的雙色熒光標(biāo)記技術(shù)，為您帶來(lái)前所未有的清晰視野。它不僅能夠直觀區(qū)分缺氧與氧化應(yīng)激狀態(tài)，還能確保實(shí)驗(yàn)結(jié)果的準(zhǔn)確性和重復(fù)性，讓您的研究數(shù)據(jù)更加信服有力。

* 可單獨(dú)檢測(cè)低氧，ROS氧化應(yīng)激，亦可同時(shí)檢測(cè)！   

高度敏感且特異的熒光探針，可測(cè)量活細(xì)胞中的缺氧和氧化應(yīng)激 適用于附著或懸浮細(xì)胞系 包括ROS和缺氧誘導(dǎo)劑在內(nèi)的完整試劑盒 Enzo Life Sciences的ROS-ID?缺氧/氧化應(yīng)激檢測(cè)套裝旨在功能性檢測(cè)活細(xì)胞（懸浮和附著細(xì)胞均適用）中的缺氧和氧化應(yīng)激水平，可使用熒光顯微鏡或流式細(xì)胞術(shù)。該套裝包括缺氧（紅色）和氧化應(yīng)激水平（綠色）的熒光探針作為兩個(gè)主要組成部分。 

實(shí)驗(yàn)原理概述：

1、缺氧檢測(cè)（紅色）：缺氧檢測(cè)試劑（Red Hypoxia Detection Reagent）是一種非熒光或弱熒光的芳香族化合物，含有硝基（NO2）基團(tuán)。在缺氧細(xì)胞中，由于存在硝基還原酶活性，硝基團(tuán)通過(guò)一系列化學(xué)反應(yīng)被轉(zhuǎn)化為羥胺（NHOH）和氨基（NH2）基團(tuán)，導(dǎo)致原始分子降解并釋放出熒光探針，從而在熒光顯微鏡下呈現(xiàn)紅色熒光。

2、氧化應(yīng)激檢測(cè)（綠色）：氧化應(yīng)激檢測(cè)試劑（Oxidative Stress Detection Reagent）為非熒光、細(xì)胞可滲透的總ROS檢測(cè)染料，能直接與多種活性氧物種反應(yīng)，如過(guò)氧化氫、過(guò)氧亞硝酸鹽和羥基自由基，生成綠色熒光產(chǎn)物，指示不同類型的ROS/RNS產(chǎn)生。此探針對(duì)超氧化物、活性氯或溴物種相對(duì)不敏感。  

產(chǎn)品亮點(diǎn)

1、精準(zhǔn)檢測(cè)，雙管齊下：該套件同時(shí)提供綠色熒光的氧化應(yīng)激檢測(cè)試劑與紅色熒光的缺氧檢測(cè)試劑，分別對(duì)應(yīng)細(xì)胞內(nèi)ROS水平與缺氧環(huán)境，實(shí)現(xiàn)精準(zhǔn)定位與定量。

2、廣泛適用，兼容性強(qiáng)：無(wú)論是貼壁細(xì)胞還是懸浮細(xì)胞，無(wú)論是采用熒光顯微鏡還是流式細(xì)胞儀，ROS-ID 套件都能靈活適應(yīng)，滿足您多樣化的實(shí)驗(yàn)需求。兩種規(guī)格（ENZ-51042-K500與ENZ-51042-0125）量身定制，靈活選擇，經(jīng)濟(jì)高效。

3、操作簡(jiǎn)便，效率提升：我們對(duì)ROS-ID? Hypoxia/Oxidative Stress Detection Mix的準(zhǔn)備過(guò)程進(jìn)行了優(yōu)化，確保實(shí)驗(yàn)準(zhǔn)備簡(jiǎn)單快捷，減少實(shí)驗(yàn)誤差，提升整體研究效率。

4、結(jié)果可視化，直觀可信：實(shí)驗(yàn)設(shè)計(jì)包含正負(fù)對(duì)照，確保每次實(shí)驗(yàn)都有基準(zhǔn)參考，使您的研究結(jié)論更具說(shuō)服力。熒光信號(hào)直觀顯示，細(xì)胞狀態(tài)一目了然，便于數(shù)據(jù)分析與解讀。  

結(jié)果分享：

圖 1. 氧化應(yīng)激（A）和低氧（B）檢測(cè)染料的吸收峰和發(fā)射峰分別為504 nm/524 nm和580 nm/595 nm。這些染料可以使用488 nm的氬離子激光器激發(fā)，并在大多數(shù)實(shí)驗(yàn)流式細(xì)胞儀的FL3通道（低氧紅染料）和FL1通道（氧化應(yīng)激染料）上檢測(cè)。 

圖 2. HeLa 細(xì)胞被種在顯微鏡載玻片上，第二天按照手冊(cè)描述，用 DFO（低氧的化學(xué)誘導(dǎo)劑）或吡咯烷酮（氧化應(yīng)激誘導(dǎo)劑）處理 4 小時(shí)，溫度為 37°C。處理后，載玻片用 PBS 洗滌，蓋上蓋玻片，并使用 Olympus BX-51 熒光顯微鏡進(jìn)行可視化觀察。

圖3. 培養(yǎng)的人類HeLa和HL-60細(xì)胞中缺氧和氧化應(yīng)激水平的檢測(cè)。細(xì)胞用缺氧誘導(dǎo)劑（DFO）和ROS誘導(dǎo)劑（花青素）處理。每個(gè)象限中的數(shù)字表示細(xì)胞（總體）的百分比。結(jié)果表明，缺氧和氧化應(yīng)激染料具有特異性。  

產(chǎn)品詳情：

應(yīng)用：流式細(xì)胞術(shù)，熒光顯微鏡，熒光檢測(cè)，高通量篩選 

應(yīng)用說(shuō)明：此試劑盒設(shè)計(jì)用于使用貼壁或懸浮細(xì)胞進(jìn)行熒光顯微鏡和/或流式細(xì)胞術(shù)。 

質(zhì)量控制：使用流式細(xì)胞術(shù)方法進(jìn)行測(cè)試，評(píng)估低氧細(xì)胞和/或具有高水平總氧化應(yīng)激的細(xì)胞，并結(jié)合染料（試劑盒中提供）。還獲得顯微鏡圖像。 

數(shù)量：對(duì)于-K500規(guī)格： 500次熒光顯微鏡分析或100次流式細(xì)胞分析。 

對(duì)于-0125規(guī)格： 125次熒光顯微鏡分析或25次流式細(xì)胞分析。 

使用/穩(wěn)定性：在適當(dāng)?shù)膬?chǔ)存條件下，試劑盒成分穩(wěn)定，直到產(chǎn)品標(biāo)簽上注明的日期。 

處理：避免凍融循環(huán)。

運(yùn)輸：干冰

短期儲(chǔ)存：-20°C 

長(zhǎng)期儲(chǔ)存：-20°C   

更多產(chǎn)品咨詢，歡迎垂詢Enzo中國(guó)區(qū)總代理，艾美捷科技！  400-6800-868，m.zihai029.com  

【附錄】發(fā)表文章：

• Cupric-ion-promoted fabrication of oxygen-replenishing nanotherapeutics for synergistic chemo and photodynamic therapy against tumor hypoxia: L. He, et al.; Acta Biomater. 162, 57 (2023), Abstract;

• Precise manipulation of circadian clock using MnO2 nanocapsules to amplify photodynamic therapy for osteosarcoma: Y.X. Ge, et al.; Mater. Today Bio 19, 100547 (2023), Abstract;

• The impact of hypoxia and oxidative stress on proteo-metabolomic alterations of 3D cholangiocarcinoma models: P. Phukhum, et al.; Sci. Rep. 13, 3072 (2023), Abstract;

• Dimethyloxalylglycine (DMOG), a Hypoxia Mimetic Agent, Does Not Replicate a Rat Pheochromocytoma (PC12) Cell Biological Response to Reduced Oxygen Culture: R. Chen, et al.; Biomolecules 12, 541 (2022), Abstract;

• Hydrogel microcapsules containing engineered bacteria for sustained production and release of protein drugs: C. Han, et al.; Biomaterials 287, 121619 (2022), Abstract;

• Inhibiting autophagy flux and DNA repair of tumor cells to boost radiotherapy of orthotopic glioblastoma: Q. Xu, et al.; Biomaterials 280, 121287 (2022), Abstract;

• Intracellular glucose starvation affects gingival homeostasis and autophagy: R. Li, et al.; Sci. Rep. 12, 1230 (2022), Abstract;

• Intrinsic radical species scavenging activities of tea polyphenols nanoparticles block pyroptosis in endotoxin-induced sepsis: Y. Chen, et al.; ACS Nano 16, 2429 (2022), Abstract;

• Iodinated cyanine dye-based nanosystem for synergistic phototherapy and hypoxia-activated bioreductive therapy: Y. Dong, et al.; Drug Deliv. 29, 238 (2022), Abstract;

• Lipoprotein-biomimetic nanostructure enables tumor-targeted penetration delivery for enhanced photo-gene therapy towards glioma: R. Wang, et al.; Bioact. Mater. 13, 286 (2022), Abstract;

• Microenvironment-driven sequential ferroptosis, photodynamic therapy, and chemotherapy for targeted breast cancer therapy by a cancer-cell-membrane-coated nanoscale metal-organic framework: W.L. Pan ,et al.; Biomaterials 283, 121559 (2022), Abstract;

• Mitochondrial glutathione depletion nanoshuttles for oxygen-irrelevant free radicals generation: A cascaded hierarchical targeting and theranostic strategy against hypoxic tumor: B. Liang, et al.; ACS Appl. Mater. Interfaces 14, 13038 (2022), Abstract;

• Multifunctional Nanosnowflakes for T1-T2 Double-Contrast Enhanced MRI and PAI Guided Oxygen Self-Supplementing Effective Anti-Tumor Therapy: Y. Lv, et al.; Int. J. Nanomedicine 17, 4619 (2022), Abstract;

• Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries: K. Haase, et al.; Biomaterials 280, 121248 (2022), Abstract;

• Platinum prodrug nanoparticles inhibiting tumor recurrence and metastasis by concurrent chemoradiotherapy: W. Jiang, et al.; J. Nanobiotechnology 20, 129 (2022), Abstract;

• Strategy for improving cell-mediated vascularized soft tissue formation in a hydrogen peroxide-triggered chemically-crosslinked hydrogel: S.Y. Wei, et al.; J. Tissue. Eng. 13, 20417314221084096 (2022), Abstract; Full Text

• A cyclic nano-reactor achieving enhanced photodynamic tumor therapy by reversing multiple resistances: P. Liu, et al.; J. Nanobiotechnology 19, 149 (2021), Abstract; Full Text

• An albumin-based therapeutic nanosystem for photosensitizer/protein co-delivery to realize synergistic cancer therapy: S.L. Ai, et al.; ACS Appl. Bio. Mater. 4, 4946 (2021), Abstract;

• An injectable hydrogel co-loading with cyanobacteria and upconversion nanoparticles for enhanced photodynamic tumor therapy: X. Zhang, et al.; Colloids Surf. B. Biointerfaces 201, 111640 (2021), Abstract;

• Biomimetic nanoreactor for targeted cancer starvation therapy and cascade amplificated chemotherapy: F. Shao, et al.; Biomaterials 274, 120869 (2021), Abstract;

• Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC: H. Chen, et al.; J. Nanobiotechnology 19, 342 (2021), Abstract; Full Text

• Combination of coumarin and doxorubicin induces drug-resistant acute myeloid leukemia cell death: N.S. Al-Abbas, et al.; Heliyon 7, e06255 (2021), Abstract; Full Text

• Encapsulating an acid-activatable phthalocyanine–doxorubicin conjugate and the hypoxia-sensitive tirapazamine in polymeric micelles for multimodal cancer therapy: X. Guo, et al.; Biomater. Sci. 9, 4936 (2021), Abstract;

• Exploiting the acquired vulnerability of cisplatin-resistant tumors with a hypoxia-amplifying DNA repair-inhibiting (HYDRI) nanomedicine: J. Chen, et al.; Sci. Adv. 7, 5267 (2021), Abstract;

• Extracellular matrix-induced GM-CSF and hypoxia Promote Immune Control of Mycobacterium tuberculosis in Human In Vitro Granulomas: A. Arbues, et al.; Front. Immunol. 12, 727508 (2021), Abstract; Full Text

• Immune/Hypoxic Tumor Microenvironment Regulation-Enhanced Photodynamic Treatment Realized by pH-Responsive Phase Transition-Targeting Nanobubbles: M. Zhao, et al.; ACS Appl. Mater. Interfaces 13, 32763 (2021), Abstract;

• Mitochondria-targeted and ultrasound-responsive nanoparticles for oxygen and nitric oxide codelivery to reverse immunosuppression and enhance sonodynamic therapy for immune activation: C. Ji, et al.; Theranostics 11, 8587 (2021), Abstract; Full Text

• MnO2-based nanomotors with active fenton-like Mn2+ delivery for enhanced chemodynamic therapy: J. Ou, et al.; ACS Appl. Mater. Interfaces 13, 38050 (2021), Abstract;

• Mucin 1 regulates the hypoxia response in head and neck cancer cells: K. Utispan, et al.; J. Pharmacol. Sci. 147, 331 (2021), Abstract;

• Ruthenium (II) complexes coordinated to graphitic carbon nitride: Oxygen self-sufficient photosensitizers which produce multiple ROS for photodynamic therapy in hypoxia: F. Wei, et al.; Biomaterials 276, 121064 (2021), Abstract;

• Stratified 3D microtumors as organotypic testing platforms for screening pancreatic cancer therapies: M.V. Monteiro, et al.; Small Methods 5, e2001207 (2021), Abstract;

• Synergy of hypoxia relief and heat shock protein inhibition for phototherapy enhancement: G. Zhang, et al.; J. Nanobiotechnology 19, 9 (2021), Abstract; Full Text

• The New Serum-Free OptiPASS ? Medium in Cold and Oxygen-Free Conditions: An Innovative Conservation Method for the Preservation of MDA-MB-231 Triple Negative Breast Cancer Spheroids: A. Goisnard, et al.; Cancers 13, 1945 (2021), Abstract; Full Text

• Biomimetic decoy inhibits tumor growth and lung metastasis by reversing the drawbacks of sonodynamic therapy: H. Zhao, et al.; Adv. Healthc. Mater. 9, e1901335 (2020), Application(s): Fluorescence microscopy using 4T1 cells, Abstract;

• Remodeling extracellular matrix based on functional covalent organic framework to enhance tumor photodynamic therapy: S.B. Wang, et al.; Biomaterials 234, 119772 (2020), Application(s): Fluorescence microscopy using CT26 cells, Abstract;

• A two-photon excited O2-evolving nanocomposite for efficient photodynamic therapy against hypoxic tumor: R.Q. Li, et al.; Biomaterials 194, 84 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract;

• Combinational phototherapy and hypoxia-activated chemotherapy favoring antitumor immune responses: B. Ma, et al.; Int. J. Nanomedicine 14, 4541 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract; Full Text

• Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells: A. Tripathi, et al.; Toxicol. Res. (Camb.) 8, 381 (2019), Application(s): Flow cytometry using granulosa cells, Abstract;

• Encircling granulosa cells protects against di-(2-ethylhexyl)phthalate-induced apoptosis in rat oocytes cultured in vitro: A. Tripathi, et al.; Zygote 27, 203 (2019), Abstract;

• Endogenous oxygen generating multifunctional theranostic nanoplatform for enhanced photodynamic-photothermal therapy and multimodal imaging: K. Wu, et al.; Theranostics 9, 7697 (2019), Application(s): Confocal microscopy using HeLa cells, Abstract; Full Text

• Glutathione depletion and dual-model oxygen balance disruption for photodynamic therapy enhancement: W. Li, et al.; Colloids Surf. B Biointerfaces 183, 110453 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract;

• Investigation of PPIX-Lipo-MnO2 to enhance photodynamic therapy by improving tumor hypoxia: L. Chudal, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 104, 109979 (2019), Application(s): Fluorescence microscopy using MCF-7 cells, Abstract;

• Laser-triggered polymeric lipoproteins for precision tumor penetrating theranostics: R. Wang, et al.; Biomaterials 221, 119413 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract;

• Monodispersed copper (I)‐based nano metal–organic framework as a biodegradable drug carrier with enhanced photodynamic therapy efficacy: X. Cai, et al.; Adv. Sci. 6, 1900848 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract; Full Text

• Oral ferroportin inhibitor ameliorates ineffective erythropoiesis in a model of β-thalassemia: V. Manolova, et al.; J. Clin. Invest. 130, 491 (2019), Application(s): Flow cytometry analysis using mouse red blood cells, Abstract;

• Oxygen-supplementing mesoporous polydopamine nanosponges with WS2 QDs-embedded for CT/MSOT/MR imaging and thermoradiotherapy of hypoxic cancer: Y. Wang, et al.; Biomaterials 220, 119405 (2019), Application(s): Fluorescence microscopy using 4T1 cells, Abstract;

• Self-generating oxygen enhanced mitochondrion-targeted photodynamic therapy for tumor treatment with hypoxia scavenging: Z. Yang, et al.; Theranostics 9, 6809 (2019), Application(s): Fluorescence microscopy using MKN-45P cells, Abstract; Full Text

• Solid matrix-supported supercritical CO2 enhances extraction of γ-linolenic acid from the cyanobacterium Arthrospira (Spirulina) platensis and bioactivity evaluation: X. Yang, et al.; Mar. Drugs 17, 203 (2019), Application(s): Fluorescence microscopy using Zebrafish larvae, Abstract; Full Text

• All-in-one theranostic nanoplatform based on hollow MoSx for photothermally-maneuvered oxygen self-enriched photodynamic therapy: J. Wang, et al.; Theranostics 8, 955 (2018), Application(s): Fluorescence microscopy using 4T1 cells, Abstract; Full Text

• Fluorinated polymeric micelles to overcome hypoxia and enhance photodynamic cancer therapy: Q. Wang, et al.; Biomater. Sci. 6, 3096 (2018), Abstract;

• High glucose-induced oxidative stress impairs proliferation and migration of human gingival fibroblasts: P. Buranasin, et al.; PLoS One 13, e0201855 (2018), Abstract; Full Text

• Light-enhanced hypoxia-responsive nanoparticles for deep tumor penetration and combined chemo-photodynamic therapy: Z. Li, et al.; Chem. Commun. 54, 13127 (2018), Abstract;

• LSD1 mediated changes in the local redox environment during the DNA damage response: M.L. Duquette, et al.; PLoS One 13, e0201907 (2018), Abstract; Full Text

• Platelet-mimicking nanoparticles co-loaded with W18O49 and metformin alleviate tumor hypoxia for enhanced photodynamic therapy and photothermal therapy: H. Zuo, et al.; Acta Biomater. 80, 296 (2018), Abstract;

• Tumor starvation induced spatiotemporal control over chemotherapy for synergistic therapy: M.K. Zhang, et al.; Small 14, e1803602 (2018), Abstract;

• Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing: A.C. Chlebowski, et al.; Toxicol. Rep. 4, 202 (2017), Application(s): Use of hypoxia detection reagent with zebrafish embryos,

• Modulation of alveolar macrophage innate response in proinflammatory-, pro-oxidant-, and infection- models by mint extract and chemical constituents: Role of MAPKs: N. Yadav & H. Chandra; Immunobiology 223, 49 (2017), Abstract;

• Nitroimidazole derivatives of polypyridyl ruthenium complexes: Towards understanding their anticancer activity and mode of action: O. Mazuryk, et al.; Eur. J. Pharm. Sci. 101, 43 (2017), Application(s): Flow cytometry analysis using HaCaT and PANC-1 cells, Abstract;

• Prodrug-embedded angiogenic vessel-targeting nanoparticle: A positive feedback amplifier in hypoxia-induced chemo-photo therapy: D. Guo, et al.; Biomaterials 144, 188 (2017), Application(s):Condocal Microscopy, Abstract; Full Text

• Tumor-penetrating nanoparticles for enhanced anticancer activity of combined photodynamic and hypoxia-activated therapy: Y. Wang, et al.; ACS Nano 11, 2227 (2017), Application(s): Flow cytometry analysis of mouse breast carcinoma cells, Abstract; Full Text

• Analysis of a nitroreductase-based hypoxia sensor in primary neuronal cultures: B.N. Lizama-Manibusan, et al.; ACS Chem. Neurosci. 7, 1188 (2016), Abstract;

• ERK2 and CHOP restrict the expression of the growth-arrest specific p20K lipocalin gene to G0: M.J. Erb, et al.; Mol. Cell. Biol. 36, 2890 (2016), Application(s): Hypoxia levels in chick embryo fibroblasts (CEF), Abstract;

• Phagocyte respiratory burst activates macrophage erythropoietin signalling to promote acute inflammation resolution: B. Luo, et al.; Nat. Commun. 7, 12177 (2016), Application(s): Flow cytometry analysis of hypoxia in exudate leukocytes and peritoneum, Abstract; Full Text

• Selective advantage of trisomic human cells cultured in non-standard conditions: S.D. Rutledge, et al.; Sci. Rep. 6, 22828 (2016), Application(s): Fluorescence microscopy on human colorectal adenocarcinoma DLD1 cells, Abstract; Full Text

• Low-level light in combination with metabolic modulators for effective therapy of injured brain: T. Dong, et al.; J. Cereb. Blood Flow Metab. 35, 1435 (2015), Application(s): Immunofluorescence Assay, Abstract; Full Text

• Quantitative measurement of redox potential in hypoxic cells using SERS nanosensors: J. Jiang, et al.; Nanoscale 6, 12104 (2014), Abstract;