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SouthernBiotech 正常山羊血清,流式細(xì)胞術(shù)、免疫組化和免疫細(xì)胞化學(xué)等多應(yīng)用研究

發(fā)布者:艾美捷科技    發(fā)布時(shí)間:2025-08-21     
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正常山羊血清取自美國來源的家養(yǎng)山羊,可用于抗體相關(guān)應(yīng)用中的對(duì)照或封閉,包括流式細(xì)胞術(shù)、免疫組化和免疫細(xì)胞化學(xué)。


作為SouthernBiotech在中國的區(qū)域總代理,艾美捷科技強(qiáng)烈推薦SouthernBiotech熱銷產(chǎn)品正常山羊血清。產(chǎn)品僅用于科研,不可用于臨床診斷。

產(chǎn)品名稱貨號(hào)詳情

正常山羊血清

0060-01查看


正常山羊血清:

來源:成年混種山羊

純化方法:經(jīng)過處理以盡量減少溶血,并經(jīng)過0.2微米過濾

緩沖液配方:含有小于0.1%的疊氮化鈉

體積:10毫升

推薦儲(chǔ)存條件:2-8°C


應(yīng)用:

封閉:1-17

對(duì)照:18

流式細(xì)胞術(shù):1-3

免疫組化-石蠟切片:4-6

免疫組化-冷凍切片:7

免疫組化-全切片:8-10

免疫細(xì)胞化學(xué):4,11-17


實(shí)驗(yàn)案例

0060-01.png

人胰腺癌細(xì)胞系MIA PaCa-2用10%正常山羊血清(SB貨號(hào)0060-01)封閉,然后用小鼠抗細(xì)胞角蛋白18-UNLB(SB貨號(hào)10085-01;右側(cè))染色,隨后用山羊抗小鼠IgG(H+L),人吸附-AF555(SB貨號(hào)1031-32)和DAPI進(jìn)行染色。


文獻(xiàn)參考:

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lineage. Immunity. 2008;29:391-403. (Block, FC)

2. Yu KO, Porcelli SA, Shuman HA. In vitro derivation of macrophage from guinea pig bone marrow with human M-CSF. J Immunol Methods.

2013;389:88-94. (Block, FC)

3. Falahat R, Wiranowska M, Gallant ND, Toomey R, Hill R, Alcantar N. A Cell ELISA for the quantification of MUC1 mucin (CD227) expressed by

cancer cells of epithelial and neuroectodermal origin. Cell Immunol. 2015;298:96-103. (Block, FC, ELISA)

4. Johnson DT, Luong R, Lee SH, Peng Y, Shaltouki A, Lee JT, et al. Deletion of leucine zipper tumor suppressor 2 (Lzts2) increases susceptibility to

tumor development. J Biol Chem. 2013;288:3727-38. (Block, IHC-PS, ICC)

5. Schopperle WM, inventor; CureMeta LLC, assignee. Systems and methods for employing podocalyxin and tra human stem cell markers as prognostic

markers for aggressive and metastatic cancer. United States patent application publication US 2014/0274773 A1. 2014 Sep 18. (Block, IHC-PS)

6. Knuth CA, Witte-Bouma J, Ridwan Y, Wolvius EB, Farrell E. Mesenchymal stem cell-mediated endochondral ossification utilising micropellets and

brief chondrogenic priming. Eur Cell Mater. 2017;34:142-61. (Block, IHC-PS)

7. Mitchell AC, Javidfar B, Bicks LK, Neve R, Garbett K, Lander SS, et al. Longitudinal assessment of neuronal 3D genomes in mouse prefrontal cortex.

Nat Commun. 2016;7:12743. (Block, IHC-FS)

8. Szabadfi K, Atlasz T, Kiss P, Reglodi D, Szabo A, Kovacs K, et al. Protective effects of the neuropeptide PACAP in diabetic retinopathy. Cell Tissue

Res. 2012;348:37-46. (Block, IHC-WM)

9. L?mmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of

cell death in vivo. Nature. 2013;498:371-5. (Block, IHC-WM)

10. Lavinsky J, Crow AL, Pan C, Wang J, Aaron KA, Ho MK, et al. Genome-wide association study identifies Nox3 as a critical gene for susceptibility to

noise-induced hearing loss. PloS Genet. 2015;11(4):e1005094. (Block, IHC-WM)

11. Lundquist P, Ritchie HH, Moore K, Lundgren T, Linde A. Phosphate and calcium uptake by rat odontoblast-like MRPC-1 cells concomitant with

mineralization. J Bone Miner Res. 2002;17:1801-13. (Block, ICC)

12. Gupta P, Han S, Holgado-Madruga M, Mitra SS, Li G, Nitta RT, et al. Development of an EGFRvIII specific recombinant antibody. BMC Biotechnol.

2010;10:72. (Block, ICC)

13. Hu H, Roth JP, Estevez CN, Zsak L, Liu B, Yu Q. Generation and evaluation of a recombinant Newcastle disease virus expressing the glycoprotein

(G) of avian metapneumovirus subgroup C as a bivalent vaccine in turkeys. Vaccine. 2011;29:8624-33. (Block, ICC)

14. Zhao W, Spatz S, Zhang Z, Wen G, Garcia M, Zsak L, et al. Newcastle disease virus (NDV) recombinants expressing infectious laryngotracheitis virus

(ILTV) glycoproteins gB and gD protect chickens against ILTV and NDV challenges. J Virol. 2014;88:8397-406. (Block, ICC)

15. Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming roadblocks are system dependent. Stem Cell

Reports. 2015;5:350-64. (Block, ICC)

16. Jana S, Hsieh AC, Gupta R. Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10

during TRAIL-induced apoptosis. Cell Death Dis. 2017;8:e3093. (Block, ICC)

17. Lima TS, Gov L, Lodoen MB. Evasion of human neutrophil-mediated host defense during Toxoplasma gondii infection. MBio. 2018;9:e02027-17.

(Block, ICC)

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seasonal and perennial allergic rhinitis. Clin Exp Allergy. 2009;39:820-8. (Control) 


SouthernBiotech(SBA)公司建于1982年,致力于為免疫研究的科學(xué)家提供各種親和純化的多克隆和單克隆二抗和其它特異性抗體,其中包括:熒光素和酶標(biāo)記物,低內(nèi)毒素/不含疊氮化物制劑,F(xiàn)(ab)和F(ab’)299片段化抗體,此外還有純化的免疫球蛋白、血清以及細(xì)胞外基質(zhì)蛋白等產(chǎn)品。作為SouthernBiotech在中國的區(qū)域總代理,艾美捷科技有限公司將為中國客戶提供全面的SouthernBiotech產(chǎn)品以及客戶訂制化服務(wù)。



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