BTLA 與 HVEM 受體（皰疹病毒進入介質(zhì)，也稱為 TNFRSF14）的結(jié)合觸發(fā)參與 T 細胞負調(diào)節(jié)的細胞信號傳導途徑。BTLA:HVEM 相互作用在炎癥、自身免疫和抗腫瘤反應的調(diào)節(jié)中發(fā)揮著關鍵作用，并且是藥物發(fā)現(xiàn)的重要靶標。BTLA:HVEM[生物素化]抑制劑篩選檢測試劑盒專為篩選和分析 BTLA:HVEM 信號傳導抑制劑而設計。該試劑盒的關鍵在于鏈霉親和素-HRP 檢測生物素標記的 HVEM 的高靈敏度。該測定只需要在微量滴定板上進行幾個簡單的步驟。首先，將 BTLA 涂覆在 96 孔板上。接下來，HVEM 與 BTLA 在板上一起孵育。最后，用鏈霉親和素-HRP 處理板，然后添加 HRP 底物以產(chǎn)生化學發(fā)光。

艾美捷 BTLA:HVEM[生物素化]抑制劑篩選檢測試劑盒：

貨號：72008

英文名稱：BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

規(guī)格：96 rxns.

同義詞：CD272、CD270、TNFRSF14、ATAR、HVEA、LIGHTR、TR2、活性檢測試劑盒、Strep HRP、鏈霉親和素

檢測試劑盒格式：化學發(fā)光

提供方式：該試劑盒采用方便的 96 孔格式，配有生物素標記的 HVEM、純化的 BTLA、鏈霉親和素標記的 HRP 以及可進行 100 次結(jié)合反應的測定緩沖液。

科學類：細胞表面受體/單次跨膜蛋白

儲存/穩(wěn)定性：按指示保存時，自收到之日起 6 個月。

應用領域：該試劑盒可用于篩選 HVEM 與 BTLA 結(jié)合的抑制劑。

運輸溫度：-80°C（干冰），避免冷凍/解凍循環(huán)。

需要但未提供的材料：

PBS（磷酸鹽緩沖鹽水）

發(fā)光計或酶標儀能夠讀取化學發(fā)光

旋轉(zhuǎn)或搖桿平臺

成分：

BTLA:HVEM[生物素化]抑制劑篩選檢測試劑盒文獻參考：

1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167.

2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.

部分引文參考：

1、Structural and Biochemical Analysis of the Dual Inhibition of MG-132 against SARS-CoV-2 Main Protease (Mpro/3CLpro) and Human Cathepsin-L

Elisa Costanzi, Maria Kuzikov

International Journal of Molecular Scienc…    |    2021 Oct 29    |    PubMed ID: 34769210    |    Read Article  

"We have measured no activity towards the second SARS-CoV-2 protease PLpro (paper in preparation).. Looking at dose-dependent MG-132 inhibition of Cathepsin-L using the BPSBioscience inhibitor screening kit, we saw a strong effect with 0.15 nM IC50 which corresponded to half of the used enzyme concentration in this biochemical assay ( ).. Previously, the inhibition of rat liver isolated Cathepsin-L was reported to be in the .."

2、Screening of Clinically Approved and Investigation Drugs as Potential Inhibitors of SARS‐CoV‐2: A Combined in silico and in?vitro Study

SerdarSerdar Durdagi Durdagi, Muge Didem Orhan

Molecular Informatics    |    2021 Sep 16    |    PubMed ID: 34529322    |    Read Article  

".. and rotigaptide) for the Spike/ACE2 interactions were ordered and tested by in vitro experiments.. Two different inhibitor screening assay kits against Spike protein were used as ACE2:SARS‐CoV‐2 Spike S1 (#79945) and SARS‐CoV‐2 Spike:ACE2 (Cat. #79931), (BPS Bioscience).. Both assays were designed for screening and profiling inhibitors of the Spike and ACE2 .."

3、Triple-target stimuli-responsive anti-COVID-19 face mask with physiological virus-inactivating agents

Werner E. G.Werner E. G. Müller Müller, MeikMeik Neufurth Neufurth

Biomaterials Science    |    2021 Jun 30    |    PubMed ID: 34190748    |    Read Article  

"A Screening Assay Kit (BPS Bioscience/Tebu-bio, Offenbach; Germany) was used as described.. In this system the recombinant ACE2 receptor (50 ng per well) was bound to .."

BPS bioscience總部坐落于世界最大之一的美國圣地亞哥生命科學研究機構(gòu)和公司集中地的中心。公司主要提供激酶、磷酸二酯酶、磷酸酶、組蛋白去甲基化酶、組蛋白甲基化轉(zhuǎn)移酶、HDACs、聚ADP核糖聚合酶。BPS公司為藥物研發(fā)用重組酶類的提供了最大選擇之一。