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CD3是T細(xì)胞受體相關(guān)CD3復(fù)合物的成員。單克隆抗體CT-3識別雞T細(xì)胞上至少三種分子量為20、19和17 kDa的多肽(其中兩種是N-糖基化的)的復(fù)合物。該抗體還從溶解了洋地黃皂苷的T細(xì)胞裂解物中共沉淀出90 kDa的非極性多肽,該非極性肽可以還原為Mr 50和40 kDa的兩種多肽。
SouthernBiotech-Pacific Blue?抗雞CD3抗體用于流式細(xì)胞術(shù)檢測。
艾美捷SouthernBiotech-小鼠抗雞CD3-PACBLU(CT-3):
貨號:8200-26
克隆:CT-3
同型:小鼠(BALB/c) IgG1κ
同型對照:小鼠IgG1-PACBLU (15H6)
特異性:雞/鴿子 CD3
其他名稱:T3/TCR復(fù)合物
免疫原:雞胸腺細(xì)胞和Ig-血液單核細(xì)胞
結(jié)合物:PACBLU (太平洋藍(lán)?)
緩沖液配方:含有< 0.1% 疊氮化鈉的磷酸鹽緩沖液
克隆性:單克隆
濃度:0.5 mg/mL
體積:0.2 mL
推薦儲存:2-8°C;避免光照
應(yīng)用:
流式細(xì)胞術(shù) – 質(zhì)量檢測 1,5,10-18
免疫組織化學(xué)-冰凍切片 – 文獻(xiàn)中有報道 2-8
免疫組織化學(xué)-石蠟切片 – 文獻(xiàn)中有報道 9
免疫沉淀 – 文獻(xiàn)中有報道 1
刺激 – 文獻(xiàn)中有報道 1

用小鼠抗雞CD3-APC(SBCat.No.8200-11)和小鼠抗雞CD8α-FITC(SB Cat.No.8220-02)對雞外周血淋巴細(xì)胞進(jìn)行染色。
SouthernBiotech小鼠抗雞CD3-PACBLU(CT-3)文獻(xiàn)參考:
1. Chen CH, Ager LL, Gartland GL, Cooper MD. Identification of a T3/T cell receptor complex in chickens. J Exp Med. 1986;164:375-80. (Immunogen, FC, IP, Stim)
2. Njenga MK, Dangler CA. Endothelial MHC class II antigen expression and endarteritis associated with Marek's disease virus infection in chickens. Vet Pathol. 1995;32:403-11. (IHC-FS)
3. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)
4. Zheng W, Yoshimura Y. Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken. J Poult Sci. 2001;38:41-9. (IHC-FS)
5. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. (FC, IHC-FS)
6. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. (IHC-FS)
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