LDN Labor Diagnostika Nord-DHEA-S ELISA：用于人血清中硫酸脫氫表雄酮（DHEA-S）直接定量測定的酶免疫測定法。

艾美捷LDN Labor Diagnostika Nord-DHEA-S ELISA（AA E-1100R）：

名稱：DHEA-S ELISA

描述：用于人血清中DHEA-S直接定量測定的酶免疫測定法。

設(shè)計：ELISA

樣本體積：25微升血清

總檢測時間：60分鐘

標(biāo)準(zhǔn)范圍：0/0.005 – 10微克/毫升

靈敏度：0.005微克/毫升

儲存條件：2 – 8°C

試劑盒：96次測定

CE認(rèn)證：是

LDN Labor Diagnostika Nord-DHEA-S ELISA檢測原理：

在未標(biāo)記的抗原（存在于標(biāo)準(zhǔn)品、對照品和樣品中）和無酶標(biāo)記抗原（綴合物）之間發(fā)生競爭，爭奪微孔板上有限數(shù)量的抗體結(jié)合位點。洗滌和傾析程序去除未結(jié)合的材料。在洗滌步驟之后，酶底物被除去。通過加入終止溶液終止酶促反應(yīng)。吸光度通過微量滴定板讀數(shù)器進行測量。所形成的顏色的強度與樣品中DHEA-S的濃度成反比。一組標(biāo)準(zhǔn)用于繪制標(biāo)準(zhǔn)曲線，從該曲線可以直接讀取DHEA-S樣品和對照的量。

LDN Labor Diagnostika Nord-DHEA-S ELISA參考文獻：

1. Chasalow FI, Blethen SL, Bradlow HL. Dehydroepiandrosterone Sulfate (DHEAS) and DHEAS-likeCompounds in Fibrocystic Disease of the Breast. Steroids. 1988; 52(3):205–15.

2. Chasalow FI, et al. Serum Levels of Dehydroepiandrosterone Sulfate as Determined by Commercial Kitsand Reagents. Steroids. 1989;54(4):373–81.

3. de Peretti E, Forest MG. Pattern of Plasma Dehydroepiandrosterone Sulfate Levels in Humans from Birthto Adulthood: Evidence for Testicular Production. J Clin Endocrinol Metab. 1978; 47(3):572–77.

4. Holtzclaw WD, Gordon GB. Measurement of Serum Levels of Dehydroepiandrosterone Sulfate: AComparison of Radioimmunoassay and Enzymatic Analysis. Steroids. 1989; 54(4): 355–71.

5. Koritnik DR, et al. A Radioimmunoassay for Dehydroepiandrosterone Sulfate in the Circulation of RhesusMonkeys. Steroids. 1983; 42(6):653–67.

6. Orentreich N, et al. Age Changes and Sex Differences in Serum Dehydroepiandrosterone SulfateConcentrations Throughout Adulthood. J Clin Endocrol Metab. 1984; 59(3):551–55.

7. Smith MR, et al. A Radioimmunoassay for the Estimationof Serum Dehydroepiandrosterone Sulphate inNormaland Pathological Sera. Clin Chim Acta. 1975; 65(1): 5–13.

8. Check JH, et al. Falsely Elevated Steroidal Assay Levels Related to Heterophile Antibodies Against VariousAnimal Species. Gynecol Obstet Invest. 1995; 40(2):139–40.

LDN Labor Diagnostika Nord GmbH & Co. KG（簡稱LDN）旨在開發(fā)、制造和向臨床和研究實驗室市場提供專業(yè)的體外診斷測試系統(tǒng)和創(chuàng)新技術(shù)。艾美捷科技是LDN Labor Diagnostika Nord的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。