Botin修飾的脫氧腺苷5'-三磷酸被廣泛用于各種非放射性DNA標(biāo)記反應(yīng)，包括缺口翻譯、隨機(jī)引物標(biāo)記、cDNA標(biāo)記和3'-末端標(biāo)記。生物素化探針已被證明與同源核酸雜交的速度和程度與非生物素化的探針相同。雜交的生物素化DNA探針可以用抗生物素蛋白和鏈霉抗生物素素蛋白檢測(cè)。Tris緩沖液（pH 7.5）中的生物素-11-dATP*1 mM*可以通過(guò)缺口翻譯、隨機(jī)引物、3'-末端標(biāo)記或PCR過(guò)程酶促摻入DNA。數(shù)字“11”是指dATP和生物素之間連接體骨架中的碳原子數(shù)。生物素和抗生物素蛋白之間更有效的相互作用將發(fā)生在較長(zhǎng)的連接體上，而較短的連接體將導(dǎo)致dATP更有效地?fù)饺隓NA。建議接頭'11'長(zhǎng)度對(duì)于大多數(shù)應(yīng)用來(lái)說(shuō)是最佳的。Tris緩沖液（pH 7.5）中的生物素-11-dATP*1 mM*在各種雜交應(yīng)用中產(chǎn)生生物素化的DNA探針，包括Southern印跡、Northern印跡、斑點(diǎn)印跡、固定細(xì)胞和組織。其化學(xué)性質(zhì)與PerkinElmer（PE）的NEL540001EA相當(dāng)。

艾美捷Biotin-11-dATP：

貨號(hào)：AAT-17014

規(guī)格：25納摩爾

危險(xiǎn)性說(shuō)明短語(yǔ)：H303, H313, H333

危險(xiǎn)符號(hào)：XN

預(yù)期用途：僅限研究使用（RUO）

風(fēng)險(xiǎn)說(shuō)明短語(yǔ)：R20, R21, R22

儲(chǔ)存：

冷凍（<-15°C）；盡量減少光照暴露

Biotin-11-dATP文獻(xiàn)參考：

Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP).

Authors: Gryder, Berkley E and Khan, Javed and Stanton, Benjamin Z

Journal: Nature protocols (2020): 1209-1236

Highly sensitive and selective electrochemical detection of Hg(2+) through surface-initiated enzymatic polymerization.

Authors: Mei, Chenyang and Lin, Dajie and Fan, Chengchao and Liu, Aili and Wang, Shun and Wang, Jichang

Journal: Biosensors & bioelectronics (2016): 105-110

Ultrasensitive electrochemical DNA sensor based on the target induced structural switching and surface-initiated enzymatic polymerization.

Authors: Wan, Ying and Wang, Pengjuan and Su, Yan and Zhu, Xinhua and Yang, Shulin and Lu, Jianxin and Gao, Jimin and Fan, Chunhai and Huang, Qing

Journal: Biosensors & bioelectronics (2014): 231-6

A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.

Authors: Wan, Ying and Xu, Hui and Su, Yan and Zhu, Xinhua and Song, Shiping and Fan, Chunhai

Journal: Biosensors & bioelectronics (2013): 526-31

Oxidative DNA injury after experimental intracerebral hemorrhage.

Authors: Nakamura, Takehiro and Keep, Richard F and Hua, Ya and Hoff, Julian T and Xi, Guohua

Journal: Brain research (2005): 30-6