C反應(yīng)蛋白（CRP）是肝臟對炎癥反應(yīng)產(chǎn)生的一種物質(zhì)。它是身體炎癥的標(biāo)志物，經(jīng)常在醫(yī)療環(huán)境中用于評估炎癥的存在和嚴(yán)重程度。CRP水平會因各種情況而升高，包括感染、損傷和慢性炎癥性疾病。

血液中高水平的CRP可以表明炎癥的存在，但不能確定確切的原因。它通常與其他臨床和實驗室發(fā)現(xiàn)結(jié)合使用，以幫助診斷和監(jiān)測感染、自身免疫性疾病和心血管疾病等疾病。CRP水平升高也可能與類風(fēng)濕性關(guān)節(jié)炎、炎癥性腸病和某些癌癥等疾病有關(guān)。

在雙抗體夾心狗/犬CRP ELISA測定中，樣品中存在的CRP與已吸附到聚苯乙烯微孔表面的抗狗CRP抗體反應(yīng)。在通過洗滌去除未結(jié)合的蛋白質(zhì)后，加入與辣根過氧化物酶（HRP）綴合的抗狗CRP抗體。這些酶標(biāo)記的抗體與先前結(jié)合的CRP形成復(fù)合物。在另一個洗滌步驟之后，通過添加顯色底物3，3’，5，5’-四甲基聯(lián)苯胺（TMB）來測定與免疫吸附結(jié)合的酶。結(jié)合酶的量直接隨測試樣品中犬CRP的濃度而變化；因此，在450nm處的吸光度是測試樣品中CRP濃度的測量值。試驗樣品中CRP的量可以根據(jù)標(biāo)準(zhǔn)曲線進(jìn)行插值，并根據(jù)樣品稀釋度進(jìn)行校正

作為Immunology Consultants Laboratory（ICL，ICL Lab）在中國的區(qū)域總代理，艾美捷科技強烈推薦ICL Lab 狗 CRP ELISA 試劑盒。產(chǎn)品僅用于科研，不可用于臨床診斷。

狗 CRP ELISA 試劑盒參數(shù)：

抗體類型：酶聯(lián)免疫吸附試驗（ELISA）

反應(yīng)性：犬/狗

宿主：雞

特異性/靶標(biāo)：C反應(yīng)蛋白（CRP）

規(guī)格：1.0

檢測范圍：3.125納克/毫升 - 200納克/毫升

靈敏度：0.855納克/毫升

檢測時間：25分鐘

樣本類型：血漿、血清、尿液

儲存條件：2-8攝氏度

文獻(xiàn)引用：

Publications citing the use of ICL's Dog CRP ELISA kit -

DeClue A. et al. Identification of Immunologic and Clinical Characteristics That Predict Inflammatory Response to C. Novyi-NT Bacteriolytic Immunotherapy. BMC Veterinary Research 14 (2018): 119. PMC. Web. 9 July 2018.

Liu DJX, Meyer E, Broeckx BJG, et al. Variability of serum concentrations of cystatin C and urinary retinol-binding protein, neutrophil gelatinase-associated lipocalin, immunoglobulin G, and C-reactive protein in dogs. J Vet Intern Med. 2018;32(5):1659-1664. doi:10.1111/jvim.15293

Sample S. et al. Use of a platelet-rich plasma-collagen scaffold as a bioenhanced repair treatment for management of partial cruciate ligament rupture in dogs. PLoS ONE 13(6): e0197204. https://doi.org/10.1371/journal.pone.0197204

Sample S. et al. Radiographic and Magnetic Resonance Imaging Predicts Severity of Cruciate Ligament Fiber Damage and Synovitis in Dogs with Cranial Cruciate Ligament Rupture. Ed. Douglas Thamm. PLoS ONE 12.6 (2017): e0178086. PMC. Web. 9 July 2018.

Defauw P, Meyer E, Duchateau L, Schoeman JP, Van de Maele I, Daminet S. Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage. J Vet Diagn Invest. 2017;29(3):346-350. doi:10.1177/1040638717695608

Muir P. et al. Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture PLoS ONE 11(8): e0159095. (2016) https://doi.org/10.1371/journal.pone.0159095

Maddens B et al. Evaluation of Kidney Injury in Dogs with Pyometra Based on Proteinuria, Renal Histomorphology, and Urinary Biomarkers J Vet Intern Med. 2011 Sep-Oct;25(5):1075-83. doi: 10.1111/j.1939-1676.2011.0772.x. Epub 2011 Aug 16.

Smets P et al. Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease JVet Intern Med 2010;24:65–72

Schoeman J et al. Assessment of renal dysfunction using urinary markers in canine babesiosis caused by Babesia rossi Veterinary Parasitology Volume 190, Issues 3–4, 21 December 2012, Pages 326–332

Hrovat A et al. Evaluation of snake envenomation induced renal dysfunction in dogs using early urinary biomarkers of nephrotoxicity The Veterinary Journal Volume 198, Issue 1, October 2013, Pages 239–244

相關(guān)產(chǎn)品：

anti-Dog CRP Antibody (Rabbit) - Antiserum

anti-Dog CRP Antibody (Mouse - 14D3) - Monoclonal

anti-Dog CRP Antibody (Mouse - Clone#14H5) - Monoclonal

Dog CRP (Native) - Purified

anti-Dog CRP Antibody (Goat) - Antiserum

ICL致力于為生命科學(xué)界生產(chǎn)高質(zhì)量的抗體、ELISA試劑盒和蛋白質(zhì)參考標(biāo)準(zhǔn)品。通過內(nèi)部開發(fā)和制造產(chǎn)品，使用內(nèi)部標(biāo)準(zhǔn)對照進(jìn)行驗證和產(chǎn)品發(fā)行，ICL在從抗原生產(chǎn)和抗體選擇到即用型試劑盒設(shè)計的所有方面都保持完全的自主權(quán)。雖然ICL的產(chǎn)品僅供研究使用，但無數(shù)的ICL抗體已成為基本體外診斷（IVD）測試的核心成分。

作為ICL在中國的區(qū)域總代理，艾美捷科技有限公司將為中國客戶提供全面的ICL以及客戶訂制化服務(wù)。