Jena bioscience--5-Methyl-CTP的應(yīng)用：

1、修飾堿基的功能影響[1-5]

2、PCR中的摻入[3]。

Cat. No. Amount

NU-1138S 10 ?l (100 mM)

NU-1138L 5 x 10 ?l (100 mM)

艾美捷5-Methyl-CTP：

貨號：NU-1138L

運(yùn)輸：使用凝膠包裝運(yùn)輸

儲存條件：需在-20°C下儲存，短期（累計不超過1周）暴露在室溫下是可能的。

保質(zhì)期：自交貨日期起12個月

分子式：C10H18N3O14P3（游離酸）

分子量：497.18 g/mol（游離酸）

精確質(zhì)量：497.00 g/mol（游離酸）

CAS號：327174-86-7（酸）

純度：≥ 95%（高效液相色譜法）

形態(tài)：水溶液

顏色：無色至微黃

濃度：100 mM - 110 mM

pH值：7.5 ±0.5

光譜特性：最大吸收波長277 nm，摩爾吸光系數(shù)9.0 L mmol^-1 cm^-1（Tris-HCl pH 7.5）

相關(guān)產(chǎn)品：

高產(chǎn)T7 RNA合成試劑盒，#RNT-101

5-Methyl-CTP參考文獻(xiàn)：

[1] Adams et al. (2014) 185 CELLULAR VIABILITY AND EXPRESSION OF GREEN FLUORESCENT PROTEIN IN BOVINE FETAL FIBROBLASTS FOLLOWING TRANSFECTION OF SYNTHETIC mRNA INCLUDING MODIFIED BASES. Reprod.Fertil. Dev. 26:207.

[2] Tchigvintsev et al. (2013) Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides. Chem. Biol. Oxford U.K. 20:1386.

[3] Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25:4169.

[4] Kormann et al. (2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology 29:154.

[5] Warren et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 (5):618.