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Molecular Depot-辣根過氧化物酶(HRP)是生物化學中應用Z廣泛的酶之一。它使用過氧化氫作為氧化劑催化多種底物的氧化。生物素化的HRP可用于擴增某些免疫測定的信號。

艾美捷 Molecular Depot-辣根過氧化物酶生物素化:
目錄編號:P2010010
包裝規(guī)格:1毫克
比活性:350 U/毫克
單位定義:在20°C的反應緩沖液中,產(chǎn)生1微摩爾的普魯士藍素從焦性沒食子的生物素化辣根過氧化物酶所需的量,反應緩沖液組成為:8毫摩爾過氧化氫,14毫摩爾磷酸鉀,0.5%焦性沒食子,pH 6.0,反應時間20秒。
生物素結合量:1.3摩爾生物素/摩爾辣根過氧化物酶(HABA測定法)
外觀:凍干粉末
儲存:-20°C或更低。避免反復凍融。
純度:>95%。
推薦緩沖液:10毫摩爾檸檬酸,pH 6.0
Molecular Depot-辣根過氧化物酶生物素化文獻參考:
False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. Piketty ML, Polak M, Flechtner I, Gonzales-Brice?o L, Souberbielle JC. Clin Chem Lab Med. 2017 May 1;55(6):780-788.
Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):205-215.
Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):216-226.
Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells. Donnenberg AD, Kanias T, Triulzi DJ, Dennis CJ, Moore LR, Meyer EM, Sinchar D, Kiss JE, Normolle DP, Gladwin MT. Cytotherapy. 2019 Jul;21(7):793-800.
Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center. Stieglitz HM, Korpi-Steiner N. Clin Biochem. 2020 Jan;75:53-61.
Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation. Muraoka A, Matsuura Y, Naitow H, Ihara M, Kunishima N. Anal Biochem. 2018 Sep 15;557:46-58.
Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children. Bogusiewicz A, Stratton SL, Ellison DA, Mock DM. Am J Clin Nutr. 2008 Nov;88(5):1291-6.
Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. McKay BE, Molineux ML, Turner RW. Methods Mol Biol. 2008;418:111-28.
Molecular Depot是一家位于加利福尼亞州圣地亞哥的創(chuàng)新生化公司。是一個一站式品牌,其中包括抗體、蛋白、試劑盒、酶、染料、脂質(zhì)、分子試劑等多種產(chǎn)品類型。專注于開發(fā)省時、經(jīng)濟高效的研究工具,以及難以找到的生物化學品。艾美捷科技是Molecular Depot的中國代理商,為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務。
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