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95個(gè)肽段組成的肽池,來源于人類冠狀病毒(HCoV)核蛋白(Nucleoprotein,瑞士蛋白庫(kù)ID:P15130)的肽段掃描(15個(gè)氨基酸長(zhǎng)度,11個(gè)氨基酸重疊)。

艾美捷PepMix HCoV-229E(NCAP)規(guī)格:
貨號(hào):PM-229E-NCAP-1
數(shù)量:每個(gè)肽段25 ?g(約15 nmol)(可用于刺激多達(dá)2.5×10?個(gè)細(xì)胞)
應(yīng)用:T細(xì)胞免疫
類別:PepMix肽段池,PepMix感染相關(guān)肽段池
條件/主題:普通感冒,新冠肺炎(COVID-19),感染,呼吸道感染
布局:冷凍干燥于玻璃瓶中
生物體:人類冠狀病毒(HCoV)
蛋白名稱:核蛋白
純度:研究級(jí):每個(gè)肽段保證為主要產(chǎn)物(HPLC/MS)
定量:無
PepMix?肽段池:
覆蓋整個(gè)蛋白的重疊肽段混合物(PepMix?)在以下應(yīng)用中極為高效:
抗原特異性T細(xì)胞刺激:用于T細(xì)胞實(shí)驗(yàn)(例如ELISpot、細(xì)胞內(nèi)細(xì)胞因子染色(ICS)、細(xì)胞介導(dǎo)的細(xì)胞毒性或增殖實(shí)驗(yàn))
體外T細(xì)胞擴(kuò)增
樹突狀細(xì)胞脈沖刺激
免疫監(jiān)測(cè)
細(xì)胞免疫反應(yīng)分析
T細(xì)胞表位鑒定
T細(xì)胞實(shí)驗(yàn)標(biāo)準(zhǔn)化
PepMix?的優(yōu)勢(shì):
每個(gè)肽段均經(jīng)過質(zhì)量控制,并保證其身份和純度
提供質(zhì)量合格證(CoA)和HPLC-MS數(shù)據(jù)
批次間一致性高
由于嚴(yán)格純化每個(gè)肽段,避免因污染缺失肽段導(dǎo)致的假陽(yáng)性T細(xì)胞反應(yīng)
由于嚴(yán)格純化每個(gè)肽段,避免因毒素抑制T細(xì)胞反應(yīng)
低生物負(fù)荷工藝,最小化內(nèi)毒素污染
實(shí)施ADCF政策
HLA獨(dú)立刺激(使用覆蓋抗原的肽段池)
文獻(xiàn)參考:
Peptide Tools to Support the Fight against COVID-19
Aude-Marie Alem1, Michael Drosch1, Ulf Reimer1, Pavlo Holenya1, Maren Eckey1, Florian Kern1,2, Holger Wenschuh1, Karsten Schnatbaum1
1 JPT Peptide Technologies GmbH, Volmerstrasse 5, 12489 Berlin, Germany. 2 Brighton and Sussex Medical School, Medical School Research
Building, Rm 109, Brighton BN1 9PS, East Sussex, UK.
To date, the understanding of the immune response to COVID-19 is still incomplete. In this application note we
describe innovative peptide-based tools for the assessment of humoral and cellular immunity to SARS-CoV-2.
These tools are suitable for immune response target identification and clinical immune monitoring. They can
also be used for T-cell and antibody response profiling and will be useful for the development of effective
diagnostics, treatments, and vaccines.
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