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染料修飾的脫氧尿苷5'-三磷酸鹽是通過(guò)常規(guī)酶摻入方法(如逆轉(zhuǎn)錄、缺口翻譯、隨機(jī)引物標(biāo)記或PCR)生產(chǎn)染料標(biāo)記DNA的最常見(jiàn)方法之一。這種酶熒光標(biāo)記方法廣泛用于FISH探針和基于微陣列的實(shí)驗(yàn)。這種iFluor 488 dUTP綴合物使用SpectrumGreen?濾光片組作為綠色熒光色(SpectrumGreen?是Vysis的商標(biāo))。與常用的綠色探針(如熒光素-12-dUTP)相比,iFluor 488提供了更亮的信號(hào),具有更強(qiáng)的光穩(wěn)定性,不受pH值的影響。
艾美捷iFluor488-dUTP:
貨號(hào) AAT-17039
單位大小 100納摩爾、25納摩爾、100納摩爾
分子量 1152.83
溶劑 水
危害語(yǔ)句代碼 H303, H313, H333
危害標(biāo)識(shí)符 XN
預(yù)期用途 僅供研究使用(RUO)
風(fēng)險(xiǎn)語(yǔ)句代碼 R20, R21, R22
儲(chǔ)存條件 冷凍(<-15°C);盡量減少光照

圖:熒光素和iFluor?488 dUTP標(biāo)記的端粒探針在中期HeLa細(xì)胞中的熒光原位雜交。
iFluor488-dUTP文獻(xiàn)參考:
Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77
Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597
SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and K?lvraa, Steen and Nielsen, Kirsten Vang and Nex?, Bj?rn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6
[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90
Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33
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