NADH，煙酰胺腺嘌呤二核苷酸，還原態(tài)，還原型輔酶Ⅰ。N指煙酰胺，A指腺嘌呤，D是二核苷酸。葡萄糖代謝時直接經(jīng)代謝所產(chǎn)生的ATP是十分的少的，而代謝產(chǎn)生的NADH或FADH2經(jīng)由一個電子傳遞與氧化磷酸反應(yīng)可產(chǎn)生大量的ATP。NADH分子是線粒體中能量產(chǎn)生鏈中的控制標(biāo)志物。NADH水平的上升指示代謝失衡的出現(xiàn)。

 

 

       監(jiān)視NAD/NADH的氧化還原狀態(tài)是表征活體內(nèi)線粒體功能的最佳參數(shù)。紫外光可以在線粒體中激發(fā)NADH產(chǎn)生熒光，用來監(jiān)測線粒體功能。一般來說，涉及到氧化還原的反應(yīng)都用得到，比如呼吸作用，光合作用，等等氨會抑制呼吸過程中的電子傳遞系統(tǒng)，尤其是NADH。因此NAD/NADH水平的監(jiān)控是能量傳遞、細(xì)胞核組織氧化還原態(tài)等研究的主要興趣點。

 

       有沒有一款可以同時檢測，NAD+,NADH，以及NAD+/NADH比率，這三個指標(biāo)的試劑盒呢？

 

        艾美捷科技特向您推薦，已被廣泛使用，發(fā)布文章不計其數(shù)的高品質(zhì)NAD/NADH比率檢測試劑盒。

 

 

       *樣品通過NAD萃取，同時檢測（NAD+NADH）與（NAD）的結(jié)果，相減計算得出NADH含量，以及NAD/NADH的比率。  

 

 

       原理：Amplite 比色法NAD/NADH比率檢測試劑盒，提供一個比色法原理的檢測培養(yǎng)細(xì)胞的總NAD/NADH，和NAD/NADH ratio的方法。在這個實驗中，裂解液中NAD可以被NAD萃取液提取，通過酶反應(yīng)被轉(zhuǎn)化為NADH，然后利用NADH探針反應(yīng)，產(chǎn)生黃色染料，此染料可以在460nm處測吸收峰，或者加入增強(qiáng)劑后，可以檢測635nm吸收峰。染料產(chǎn)生顏色與細(xì)胞裂解液中NAD 或者NADH的濃度呈正比。  

 

 

《卓越的標(biāo)準(zhǔn)曲線》

 

《發(fā)表文章結(jié)果：Ren, T., et al. Oncogene  36.42(2017).》

   

 

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