- 中文名稱
Endothelial Cell Adhesion Molecule (ESAM) BioAssay ELISA Kit (Human)
- 英文名字
- Endothelial Cell Adhesion Molecule (ESAM) BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 024798
- 產品報價
- ￥1/48Tests ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Human Endothelial Cell Adhesion Molecule (ESAM) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ESAM in human tissue homogenates, cell lysates and other biological fluids.Detection Range:0.312-20ng/mlSensitivity:0.132ng/mlPrecision:Intra-Assay: CV<10%Inter-Assay: CV<12%Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to ESAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ESAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ESAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ESAM in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*024798A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*024798B: Standard, 2x1vial 024798C: Standard Diluent, 1x20ml*024798D: Detection Reagent A, 1x120ul *024798E: Detection Reagent B, 1x120ul 024798F: Assay Diluent A, 1x12ml024798G: Assay Diluent B, 1x12ml024798H: TMB Substrate, 1x9ml024798K: Stop Solution, 1x6ml024798L: Wash Buffer, 30x, 1x20ml Storage and Stability:Store *024798A, *024798B, *024798D and *024798E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
- 產品特點
- 針對Endothelial Cell Adhesion Molecule (ESAM) BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Endothelial Cell Adhesion Molecule (ESAM) BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
