- 中文名稱
FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)
- 英文名字
- FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)
- 供應商
- USBiological
- 產品貨號
- 029475
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- FGF-23, a phosphate-regulating hormone, plays a critical role in phosphorus homeostasis. The study of FGF-23 is important in understanding the role it plays in such processes as bone remodeling and kidney function. FGF-23 is responsible for the inhibition of renal tubular reabsorption of inorganic phosphorus and, consequently, the regulation of phosphorus homeostasis. FGF-23 mRNA is expressed in the brain, in the thymus at low levels, in dendritic cells, with the highest expression in osteocytes and osteoblasts during phases of active bone remodeling. Because circulating levels of FGF-23 are regulated by vitamin D and phosphorus, normal skeletal and soft tissue development require vitamin D and a functional vitamin D signaling pathway. Diseases resulting from aberrant FGF-23 expression include inherited forms of rickets, certain kidney diseases and phosphorus-related tumorsIntended Use: FGF-23, Rat, BioAssay ELISA Kit is used for the non-radioactive quantification of FGF-23 in rat serum, plasma, and tissue culture samples. This kit specifically measures native rat FGF-23. One kit is sufficient to measure 41 unknown samples in duplicate. This kit is for research use only.Detection Range:0.82-20ng/mlSensitivity:0.05ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity:The antibody pair used in this assay is specific to rat FGF-23 and does not significantly crossreact to the following molecules/hormones tested: other FGFsPrinciple of the Test:This assay is a Sandwich ELISA based, sequentially, on: 1) concurrent capture of rat FGF-23 molecules from samples to the wells of a microtiter plate coated with a polyclonal rabbit anti-FGF-23 antibody, and binding of a second biotinylated polyclonal goat anti-FGF-23 antibody to the captured molecules, 2) washing of unbound materials from samples, 3) binding of streptavidin-horseradish peroxidase conjugate to the immobilized biotinylated antibodies, 4) washing of excess of free enzyme conjugates, and 5) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3’,5,5’-tetramethylbenzidine. The enzyme activity is measured spectrophotometrically by the increased absorbance at 450–590nm after acidification of formed products. Since the increase in absorbance is directly proportional to the amount of captured rat FGF-23 in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of rat FGF-23.Kit Components:029475A: FGF-23 ELISA Microtiter Strips, 1x96 wells029475B: HRP Wash Buffer Concentrate, 10X, 2x50ml029475C: FGF-23 Standard, 1x1 vial029475D: FGF-23 Quality Control 1, 0.22-0.46ng/ml, 1x1 vial029475E: FGF-23 Quality Control 2, 1.9-4.0ng/ml, 1x1 vial029475F: Matrix Solution, 1x1.5ml029475G: Assay Buffer, 1x12ml029475H: FGF-23 Detection Antibody, 1x12ml029475J: Streptavidin (HRP), 1x12ml029475K: TMB Substrate, 1x12ml029475L: Stop Solution, 1x12mlStorage and Stability:Recommended storage for kit components is 4°C. Stable for 6 months from the date of receipt. All components are shipped and stored at 4°C. Reconstituted standards and controls can be frozen for future use, but repeated freeze thaws should be avoided. Do not mix reagents from different kits.
- 產品特點
- 針對FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到FGF-23, Rat, BioAssay ELISA Kit (Fibroblast Growth Factor 23, Fgf23)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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