中文名稱

Monosodium Urate (MSU), crystals

英文名字

Monosodium Urate (MSU), crystals

供應商

USBiological

產品貨號

045038

產品報價

￥1/2mg

產品說明書

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購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料

USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述

NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity. Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1beta and IL-18.Appearance:Solid crystalsEndotoxin: ≤ 0.001EU/ug purified protein (LAL method)Solubility:Not soluble in PBS. Reconstitute 2mg of MSU in 100ul PBS to obtain a 20mg/ml suspension.Activation of THP-1 cells by uric acid:1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovineserum (FCS), 50uM B-mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown in suspension to a density of 1.5x10e6 cells/ml in tissue culture flasks.2. Treat THP-1 cells for 3 hours with 0.5uM PMA the day before stimulation.3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x10e5 cells/well in RPMI medium.4. The next day, wash cells with PBS and add 500ul Optimem (Invitrogen).5. Stimulate the cells for 6 hours by addition of 50-300ug/ml of the suspension of uric acid (vortex the suspension just before use).6. As a positive control, 5mM of ATP is incubated for 6 hours.Detection of the mature Caspase-1 in the supernatant of uric acid-activated THP-1 cells:1. Collect the supernatant of each well (500ul). Add 500ul methanol and 125ul chloroform.2. Vortex and centrifuge for 5 min at 13,000 rpm.3. Remove the upper phase.4. Add 500ul methanol and centrifuge for 5 min at 13,000 rpm.5. Remove the supernatant, dry the pellet for 5 min at 50°C.6. Add 60ul of 1X loading buffer and DTT (final concentration of 0.1M).7. Mix and boil before loading on a 15% SDS-gel and transfer onto nitrocellulose membrane. The mature Caspase-1 is detected using the Caspase-1 (p20) (human), mAb (Bally-1) antibody (Cat No 044627).Storage and Stability:May be stored at 4oC for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

產品形式

艾美捷向您提供的Monosodium Urate (MSU), crystals產品形式查看產品說明書，建議按照產品說明書進行應用操作。

保存建議

接收到Monosodium Urate (MSU), crystals產品后，置于-20°C溫度下保存。

其他

Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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