NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death.? In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB.? In turn IkB is phosphorylated by a cellular kinase complex called IKK.? IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus.? IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1.? Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
應用類型
ELISA,Immunohistochemistry,Western Blot,
免疫原
IKK a peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
來源宿主
Rabbit
反應性
H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)
保存建議
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
