中文名稱

原兒茶酸二加氧酶（25mg）

英文名字

Protocatechuate Dioxygenase

供應商

Cayman Chemical

產品貨號

10011353-25

產品報價

￥已停產

產品說明書

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購買方式

銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.

產品新聞

背景資料

Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2.{15534} The PCD holoenzyme consists of α (23 kDa) and β (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.{15536} Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex.{15534} PCD is an important component of bacterial metabolism of aromatic compounds found in the soil. However, many publications are now reporting the usefulness of the PCD/PCA oxygen scrubbing (oxygen scavenging) system for maintaining anaerobic experimental conditions.{15535,15565} Cayman’s protocatechuate dioxygenase is the 3,4-PCD expressed and purified from a bacterial overexpression system. 3,4-PCD catalyzes the oxidative ring cleavage of 3,4-dihydroxybenzoate to produce β-carboxy-cis,cis-muconate. Activity of the PCD can be measured by monitoring the rate of O2 uptake with an oxygen electrode, or by measuring the amount of product (β-carboxy-cis,cis-muconate) formed at 290 nm.

產品描述

提供的原兒茶酸二加氧酶（25mg）產品形式為：A lyophilized powder from a solution of 20 mM potassium phosphate, pH 7.2, containing 100 mM sodium chloride.

產品形式

Protocatechuate dioxygenase (PCD) catalyzes the proximal extradiol ring cleavage of protocatechuate (PCA) with attendant incorporation of both atoms of oxygen from O2.{15534} The PCD holoenzyme consists of ?? (23 kDa) and ?? (26.5 kDa) subunits, which form a tetramer with four ferric ions for a total weight of ~200 kDa.{15536} Iron is essential for activity because the substrate, PCA, initially binds directly to the iron through both hydroxyl groups to form a chelate complex.{15534} PCD is an important component of bacterial metabolism of aromatic compounds found in the soil. However, many publications are now reporting the usefulness of the PCD/PCA oxygen scrubbing (oxygen scavenging) system for maintaining anaerobic experimental conditions.{15535,15565} Cayman??s protocatechuate dioxygenase is the 3,4-PCD expressed and purified from a bacterial overexpression system. 3,4-PCD catalyzes the oxidative ring cleavage of 3,4-dihydroxybenzoate to produce ??-carboxy-cis,cis-muconate. Activity of the PCD can be measured by monitoring the rate of O2 uptake with an oxygen electrode, or by measuring the amount of product (??-carboxy-cis,cis-muconate) formed at 290 nm.

保存建議

建議您在收到Cayman Chemical品牌的原兒茶酸二加氧酶（25mg）產品后置于-80度保存，產品有效期為180天.

其他

Cayman Chemical向全球科學工作者提供多研究領域的生化、免疫試劑和分析試劑盒，其產品被廣泛應用于腫瘤、氧化氮、神經學、凋亡、氧化性損傷、內分泌學等不同研究領域.Cayman Chemical可提供用于檢測的特色試劑盒，如：類花生酸類物質、游離的生物標志、環核苷、激素及氧化氮等；也提供多種高質量試劑，包括：類花生酸類物質、氧化氮試劑及許多相關脂質、脂肪酸、酶和抗體.作為Cayman Chemical在中國的區域總代理，艾美捷科技有限公司將為中國客戶提供最全面的Cayman Chemical產品以及客戶訂制化服務.

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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