- 中文名稱
Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit
- 英文名字
- Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- 144064
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2 The standard product used in this kit is recombinant rat TNFα, consisting of 157 amino acids with the molecular mass of 17kD.Principle of the Assay:The Tumor Necrosis Factor alpha (TNFa), BioAssay ELISA Kit (Rat) is based on standard sandwich enzyme-linked immunosorbent assay technology. A mouse monoclonal antibody specific for TNFa has been pre-coated onto 96-well plates. Standards (E. coli-expressed recombinant fragment corresponding to L80 thru L235 of rat TNFa) and test samples are added to the wells, incubated and then biotinylated detection goat polyclonal antibody specific for TNFa is added, followed by an incubation step and a washing step with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP-mediated reaction. TMB is catalyzed by HRP to produce a blue colored product that changes into yellow upon addition of acidic stop solution. The intensity of the yellow color is proportional to the amount of rat TNFa in the sample.This kit is intended for the quantitative determination of rat TNFa in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).Detection Range:15.6pg/ml-1000pg/mlSensitivity:< 1 pg/mlPrecision:Intra-assay: <10% (CV)Inter-Assay: <10% (CV)Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:144064A: Microtiter Plate, 1x96wells. Pre-coated with anti-rat TNFa antibody144064B: TNFa standard, 2x10ng144064C: Anti-rat TNFa (Biotin), 1x130ul (dilute 1:100)143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilute 1:100)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability: Unopened kit is stable at 4°C for 6 months.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 min. Do not wash. 2. Add biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min.5. Add Stop solution and read absorbance at 450nm.
- 產品特點
- 針對Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Tumor Necrosis Factor alpha (TNFa), Rat, BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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