- 中文名稱
Insulin (INS) BioAssay ECL Kit (Rat)
- 英文名字
- Insulin (INS) BioAssay ECL Kit (Rat)
- 供應商
- USBiological
- 產品貨號
- 157756
- 產品報價
- ￥1/48Test ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Insulin (INS) BioAssay ECL Kit (Rat) is a quantitative, in vitro, competitive inhibition immunoassay (ECL) suitable for use in Serum, plasma and other biological fluids.Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (in standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Insulin (INS) level in the sample or standard.Detection Range:246.91-20000pg/mlSensitivity:The minimum detectable dose of this kit is typically less than 26.3pg/ml.Specificity:This competitive assay has high sensitivity and excellent specificity for detection of Insulin (INS). No significant cross-reactivity or interference between Insulin (INS) and analogues was observed. Stability:The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Precision:Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%Kit Components:Microtiter Plate, 1x96 wells, Pre-coated, ready to use,?well strip platePlate sealer for 96 wellsStandard, 2x1 vialStandard Diluent, 1x20mlDetection Reagent A, 1x120ulDetection Reagent B, 1x120ulAssay Diluent A, 1x12mlAssay Diluent B, 1x12mlSubstrate A, 1x10mlSubstrate B, 1x2mlWash Buffer, 30X, 1x20mlMaterials Required but not Supplied:1. Luminometer capable of reading 96-well microplates with the following parameters:?lag time 30.0secs; read time 1.0 sec/well.2. Precision single or multi-channel pipettes and pipette tips with disposable tips.?3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionProtocol Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C.8. Read RLU value immediately.Sample Collection:Serum:Use a serum separator tube and allow samples to clot for two hours at RT or overnight at 4°C before centrifugation for 20 minutes at approximately 1000×g. Assay freshly?prepared serum?immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at?1000×g at 2-8°C within 30 minutes of collection.Remove plasma and assay immediately?or store samples?in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Other Biological Fluids:Centrifuge samples for 20 minutes at 1000xg. Remove particulates and assay?immediately or store samples in aliquot at -20°C or -80°C for later use.Avoid repeated?freeze/thaw cycles.Note:Samples to be used within 5 days may be stored at 4°C, otherwise samples must be stored at -20°C ( 1?month) or -80°C ( 2 months) to avoid loss of bioactivity and contamination.Sample hemolysis will influence the result, so hemolytic specimens cannot be detected.When performing the assay, bring samples to room temperature.Storage and Stability:1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.3. Expiration date is 6 months from shipment date.4. It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
- 產品特點
- 針對Insulin (INS) BioAssay ECL Kit (Rat)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Insulin (INS) BioAssay ECL Kit (Rat)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
