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中文名稱

C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)

英文名字
C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)
供應商
USBiological
產品貨號
167774
產品報價
¥1/100Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat) is used for quantitative protein detection, establishing normal range and validation of antibody array results.Sample Type:Serum, plasma and cell culture media etc.Specificity:The capture antibody provided in this kit recognizes human, mouse and rat C-Peptide. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.Sensitivity:The intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. Range:The detection range for the kit is 0.1-1000ng/mlTest Principle:C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. The minimum detectable concentration of C-Peptide is 772pg/ml. Kit Components:C-Peptide Microplate (Item A), 1x96wellsWash Buffer Concentrate (Item B), 1x25mlStandard C-Peptide peptide (Item C), 2x vialsAssay Diluent A (Item D), 1x30mlAssay Diluent B (Item E), 1x15 mlBiotinylated C-Peptide peptide (Item F), 2x vialsHRP-Streptavidin concentrate (Item G), 1x600ulTMB One-Step Substrate Reagent (Item H), 1x12mlStop Solution (Item I), 1x8mlPositive control (Item M), 1x vialAnti- C-Peptide polyclonal antibody (Item N), 2x vialsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到C-Peptide BioAssay ELISA Kit (Human, Mouse, Rat)產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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