Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. The basic structure is a 146 bp strand of DNA wrapped around a histone octamer containing pairs of the four core histones (H2A, H2B, H3, and H4). Histones are subjected to numerous post-translational modifications including methylation. Histone lysine methylation is an important post-translational modification linked to diverse biological pathways including gene transcription, heterochromatin maintenance, and double stranded DNA break repair.{20250} Most lysine methylation events are carried out by a SET domain containing HMTase, and methylation at lysine 9 of histone 3 (H3K9) is important for the recruitment of the heterochromatin protein 1 and the establishment of heterochromatin.{20252,17726,29238} The SET1/MLL (KMT2) family of methyltransferases is the principal enzyme family responsible for H3K9 methylations.{19551,19556} The WDR5/RbBP5/Ash21/DPY-30 subcomplex is required for the H3K9 dimethylation activity of the MLL1 core complex.{20268}
應用類型
ELISA
免疫原
Synthetic peptide from human Histone H3 aa 1-30 (di-methyl K9) conjugated to KLH
