- 中文名稱
Glycine Amidinotransferase, Mitochondrial (GATM) BioAssay ELISA Kit (Mouse) discontinued
- 英文名字
- Glycine Amidinotransferase, Mitochondrial (GATM) BioAssay ELISA Kit (Mouse) discontinued
- 供應商
- USBiological
- 產品貨號
- 193265
- 產品報價
- ￥1/48Tests ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
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- 背景資料
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- 產品描述
- The BioAssay?ELISA Kit is a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of Glycine Amidinotransferase, Mitochondrial (GATM) (Mouse). This kit for research use only, not for therapeutic or diagnostic applications.Kit Detection Range:100-2500pg/mLKit Sensitivity:1.0pg/mLAssay Principle:Glycine Amidinotransferase, Mitochondrial (GATM) BioAssay ELISA Kit (Mouse) utilizes the quantitative Competitive Enzyme Immunoassay technique,?utilizing a monoclonal antibody and an conjugate to Glycine Amidinotransferase, Mitochondrial (GATM) (Mouse). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since?Protein?from samples and?Protein-HRP conjugate compete for the anti-Protein?antibody binding site. Since the number of sites is limited, as more sites are occupied by?Protein?from the sample, fewer sites are left to bind the?Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The?Target Protein?concentration in each sample is interpolated from this standard curve.Materials and Equipment Supplied:1. Microtiter Plate, 96 wells, stripwell2. Enzyme Conjugate, 6mL, 1 vial3. , 1 vial4. Standard B, 50pg/mL, 1 vial5. Standard C, 100pg/mL, 1 vial6. Standard D, 250pg/mL, 1 vial7. Standard E, 500pg/mL, 1 vial8. Standard F, 1000pg/mL, 1 vial9. Substrate A, 6mL, 1 vial10. Substrate B, 6mL, 1 vial11. Stop Solution, 6mL, 1 vial12. Wash Solution (100X), 10mL, 1 vial13. Balance Solution, 3mL, 1 vial14. Protocol Manual?Materials and Equipment Required, but not Supplied:1.?Precision pipettors and disposable tips to deliver 10-1000uL. A multi-channel pipette is desirable for large assays.2.?100mL and 1 Liter graduated cylinders.3.?Distilled or deionized water.4.?Tubes to prepare sample dilutions.5.?Absorbent paper.6.?Microplate reader capable of measuring absorbance at 450nm.7.?Centrifuge capable of 3000×g.8.?Microplate washer or washing bottle.9.?Incubator (37°C).10.?Data analysis and graphing software.4.? Specimen Collection and Storage:Sample Type I: serum or plasmaSample Type II: cell culture?supernatants, body fluid and tissue homogenatea. Serum:Use a serum separator tube and allow samples to clot for 2 hours at room temperature or overnight at 2-8°C. Centrifuge at approximately 1000 × g (or 3000rpm) for 15 minutes. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°Cb. Plasma:Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g (or 3000rpm) at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C.c. Tissue Homogenates:The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS (0.02mol/L, pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in a certain amount of PBS?with a glass homogenizer on ice. The resulting suspension was subjected to ultrasonication or to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 15 minutes at 1500×g (or 5000rpm). Remove the supernate and assay immediately or aliquot and store samples at -20°C or -80°C.d. Cell Lysates:Cells should be lysed according to the following directions-1.?Adherent cells should be detached with trypsin and then collected by centrifugation. Suspension cells can be collected by centrifugation directly.2.?Wash cells three times in PBS.3.?Cells were resuspended in PBS and subjected to ultrasonication for 3 times. Alternatively, freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.4.?Centrifuge at 1000×g (or 3000rpm) for 15 minutes at 2-8 °C to remove cellular debris.5.?Assay immediately or store samples at -20°C or -80°C.e. Cell Culture Supernatants and other Body fluids:Centrifuge cell culture media at 1000×g (or 3000rpm) for 15 minutes to remove debris. Assay immediately or store samples at -20°C or -80°C.?Simple Protocol:Please read Reagent Preparation above before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the microtiter plate.1. Secure the desired numbers of coated wells in the holder then add 100uL of Standards or Samples to the appropriate well in the antibody pre-coated Microtiter Plate. Add 100uL of PBS (pH 7.0-7.2) in the blank control well.2. Dispense 10uL of Balance Solution into 100uL specimens, mix well.Note: This step is required when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then this step should be skipped.3. Add 50uL of Conjugate to each well (NOT blank control well). Mix well. Mixing well in this step is important. Cover and incubate the plate for 1 hour at 37°C.4. Wash the microtiter plate using one of the specified methods indicated (manual or automatic).5. Add 50uL Substrate A and 50uL Substrate B to each well including blank control well, subsequently. Cover and incubate for 10-15 minutes at 20-25°C. (Avoid sunlight).6. Add 50uL of Stop Solution to each well including blank control well. Mix well.7. Determine the Optical Density (O.D.) at 450nm using a microplate reader immediately.Typical Certificate of Analysis:Important! Standard curve for demonstration only. Please refer to lot-specific Kit Protocol.1. Same Lot CV%: 4.4, 5.62. Different Lot CV%: 6.6, 7.93. Spike Recovery: 94-103%4. LinearityRange %1:296-? 1011:493 - 1071:892 - 1001:1696 - 1085. Sensitivity: 1.0 pg/mL.6. Specificity: This assay has high sensitivity and excellent specificity for detection of the target antigen. No significant cross-reactivity or interference with analogues was observed.?Note: Cross-reactivity detection between the target antigen and all analogues has not been determined and cross-reaction may exist in some cases.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support:?tech@usbio.netCustomer Service: service@usbio.netTel:?781-639-5092 ??800-520-3011Fax:?978-594-8052
- 產品特點
- 針對Glycine Amidinotransferase, Mitochondrial (GATM) BioAssay ELISA Kit (Mouse) discontinued該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Glycine Amidinotransferase, Mitochondrial (GATM) BioAssay ELISA Kit (Mouse) discontinued產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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