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中文名稱

Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) discontinued

英文名字
Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) discontinued
供應商
USBiological
產品貨號
196455
產品報價
¥1/48Tests   ¥1/96Tests
產品說明書
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購買方式
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背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) s a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of Mast Cell Tryptase (MCT) in rouse serum, plasma, cell culture supernatants, body fluid and tissue homogenates.Detection Range:0.5-10ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity: This assay has high sensitivity and excellent specificity for detection of MCT (Rat). No significant crossreactivity or interference with analogs was observed.?Note: Crossreactivity detection between the MCT (Rat) and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:196455A: Microtiter Strips, 1x48 or 1x96 wells196455B: Enzyme Conjugate, 1x3ml or 1x6ml196455C: Standard, 0ng/ml, 1x1 vial196455D: Standard, 0.5ng/ml, 1x1 vial196455E: Standard, 1ng/ml, 1x1 vial196455F: Standard, 2.5ng/ml, 1x1 vial196455G: Standard, 5ng/ml, 1x1 vial196455H: Standard, 10g/ml, 1x1 vial196455J: Substrate A, 1x6ml196455K: Substrate B, 1x6ml196455L: Stop Solution, 1x6ml196455M: Wash Solution (100X), 1x10ml196455N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) utilizes the quantitative Competitive Enzyme Immunoassay technique,?utilizing a monoclonal antibody and an conjugate to Mast Cell Tryptase (MCT) (Rat). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since?MCT?from samples and?MCT-HRP conjugate compete for the anti-MCT?antibody binding site. Since the number of sites is limited, as more sites are occupied by?MCT?from the sample, fewer sites are left to bind the?MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The?MCT?concentration in each sample is interpolated from this standard curve.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.net
產品特點
針對Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) discontinued該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Mast Cell Tryptase (MCT) BioAssay ELISA Kit (Rat) discontinued產品后將其置于4°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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