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中文名稱

Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit (Human) discontinued

英文名字
Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit (Human) discontinued
供應商
USBiological
產品貨號
197826
產品報價
¥1/48Tests   ¥1/96Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit is a 1.5 hour solid-phase competitive ELISA designed for the quantitative determination of Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) in human serum, plasma, cell culture supernatants, tissue homogenates and body fluids. This kit for research use only, not for therapeutic or diagnostic applications.Detection Range:0.5-10ng/mlSensitivity:0.1ng/mlPrecision:Intra-Assay %CV: <10Inter-Assay %CV: <10Specificity:This assay has high sensitivity and excellent specificity for detection of Nrf2. No significant crossreactivity or interference with analogs was observed.Note: Crossreactivity detection between Nrf2 and all analogs has not been determined and crossreaction may exist in some cases.Assay Principle:Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit (Human) utilizes the quantitative Competitive Enzyme Immunoassay technique,?utilizing a monoclonal antibody and a conjugate to Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) (Human). The assay sample and buffer are incubated together with the HRP conjugate in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Target Protein concentration since?Protein?from samples and?Protein-HRP conjugate compete for the anti-Protein?antibody binding site. Since the number of sites is limited, as more sites are occupied by?Protein?from the sample, fewer sites are left to bind the?Protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The?Target Protein concentration in each sample is interpolated from this standard curve.Kit Components:197826A: Microtiter Strips, 1x48 wells or 1x96 wells197826B: Enzyme Conjugate, 1x3ml or 1x6ml197826C: Standard, 0ng/ml, 1x1 vial197826D: Standard, 50ng/ml, 1x1 vial197826E: Standard, 100ng/ml, 1x1 vial197826F: Standard, 250ng/ml, 1x1 vial197826G: Standard, 500ng/ml, 1x1 vial197826H: Standard, 1000ng/ml, 1x1 vial197826J: Substrate A, 1x6ml197826K: Substrate B, 1x6ml197826L: Stop Solution, 1x6ml197826M: Wash Solution, 100X, 1x10ml197826N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Note: The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma,?then the BALANCE SOLUTION is a superfluous reagent.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support:?tech@usbio.netCustomer Service: service@usbio.netTel:?781-639-5092 ??800-520-3011Fax:?978-594-8052
產品特點
針對Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit (Human) discontinued該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Nuclear Factor Erythroid 2-related Factor 2 (Nrf2) BioAssay ELISA Kit (Human) discontinued產品后將其置于4°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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