Anti Zic-1 Antibody recognizes the product of the Zic-1 gene, that encodes a zinc finger protein which is expressed in the developing or matured central nervous system in a highly restricted manner.? Zic-1 is expressed in granule cells that make synaptic contact with Purkinje cells.? Clearly Zic-1 is a gene critical to cerebellar pattern formation.? The expression of Zic genes is first detected at gastrulation and at neurulation, becomes restricted to the dorsal neural ectoderm and the dorsal paraxial mesoderm.? Zic-2 and Zic-3 are highly similar genes, especially in their product's zinc finger motif and by comparison of their genomic organization in that they share common exon-intron boundaries and belong to the same gene family.? By comparison in function, Zic-2 is essential for the formation of the brain and Zic-3 is important for right and left axis formation.? The Zic-1 gene has been mapped to chromosome 9 in mouse.? The 5' flanking region of the Zic-1 gene contains a region-specific enhancer determined to be essential in in vivo and in vitro deletion analysis.? The temporal profile of mRNA expression differs for each of the Zic gene products.? The Drosophila odd-paired gene is highly homologous to the Zic gene family.
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with an 18 aa synthetic peptide from a region near the N-Terminus of mouse Zic-1. This domain is completely conserved in human ZIC-1.
來源宿主
Rabbit
反應性
H. sapiens (Human); Mus musculus (Mouse)
保存建議
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
