Anti-Malate dehydrogenase recognizes the protein malate dehydrogenase, an oxidoreductase that catalyzes the oxidation of malate to oxaloacetate with the concomitant reduction of NAD+ to NADH. This reaction occurs in various metabolic pathways, the most notable being the citric acid cycle and gluconeogenesis. Two isoforms of malate dehyrogenase exist in eukaryotes localized in either the cytoplasm or the mitochondrial matrix.
應用類型
ELISA,Immunoprecipitation,Western Blot,
免疫原
Malate Dehydrogenase [Pig Heart]
來源宿主
Sheep
反應性
Sus scrofa domesticus (Pig, Porcine, Swine)
保存建議
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
