- 中文名稱
Tumor Necrosis Factor Alpha (TNFα) BioAssay ELISA Kit (Porcine) discontinued
- 英文名字
- Tumor Necrosis Factor Alpha (TNFα) BioAssay ELISA Kit (Porcine) discontinued
- 供應商
- USBiological
- 產品貨號
- 205920
- 產品報價
- ￥1/48Tests ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Porcine Tumor Necrosis Factor alpha (TNFa) ELISA Kit is a 1.5 hour solid-phase Competitive ELISA designed for the quantitative determination of Tumor Necrosis Factor Alpha in serum, plasma, cell culture supernatants, cell lysates, body fluid and tissue homogenates.Detection Range:50-1000pg/mlSensitivity:1.0pg/mPrecision:Intra-Assay CV: <10%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor alpha (TNFa) (Porcine). No significant crossreactivity or interference with analogs was observed.?Note: Crossreactivity detection between the TNFa and all analogs has not been determined and crossreaction may exist in some cases.Assay Principle:This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that has been pre-coated with a monoclonal anti-TNFa antibody. The assay samples and standards are incubated together with a TNFa protein-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between TNFa-HRP conjugate and TNFa protein in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFa concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The TNFA concentration in each sample is interpolated from this standard curve.Kit Components:205920A: Microtiter Strips, 1x96 wells, stripwell205920B: Enzyme Conjugate, 1x6ml 205920C: Standard, 0pg/ml, 1x1vial205920D: Standard, 50pg/ml, 1x1vial205920E: Standard, 100pg/ml, 1x1vial205920F: Standard, 250pg/ml, 1x1vial205920G: Standard, 500pg/ml, 1x1vial205920H: Standard, 1000pg/ml, 1x1vial205920J: Substrate A, 1x6ml205920K: Substrate B, 1x6ml205920L: Stop Solution, 1x6ml205920M: Wash Solution (100X), 1x10ml205920N: Balance Solution, 1x3mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support:?tech@usbio.netCustomer Service: service@usbio.netTel:?781-639-5092 ??800-520-3011Fax:?978-594-8052
- 產品特點
- 針對Tumor Necrosis Factor Alpha (TNFα) BioAssay ELISA Kit (Porcine) discontinued該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Tumor Necrosis Factor Alpha (TNFα) BioAssay ELISA Kit (Porcine) discontinued產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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