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細胞分析試劑盒
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中文名稱

ANGPTL3, BioAssay ELISA Kit (Mouse, Rat) (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5)

英文名字
ANGPTL3, BioAssay ELISA Kit (Mouse, Rat) (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5)
供應商
USBiological
產品貨號
207053
產品報價
¥1/96Tests
產品說明書
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背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Seven proteins containing an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, both characteristics of angiopoietins, have been identified and therefore designated angiopoietin-like proteins 1-7 (ANGPTL 1-7). ANGPTL3 and ANGPTL6 (Angiopoietin-related growth factor; AGF), among others, function to regulate angiogenesis and also appear to directly regulate lipid, glucose, and energy metabolism. ANGPTL3 plasma levels are increased in a mouse model of diabetes mellitus.Sample Type:Cell culture supernatant, plasma, serumIntended Use:The ANGPTL3, mouse/rat, BioAssay Dual ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat ANGPTL3 in serum, plasma and cell culture supernatant. Sensitivity:15pg/mlRange:0.016-1ng/mlSpecificity:Recognizes mouse and rat ANGPTL3. Does not cross-react with human ANGPTL3, mouse ANGPTL4 or human ANGPTL4.Test Principle:This BioAssay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.Kit Components:Plate coated with mouse ANGPTL3 antibody, 1x96wellsWash Buffer 10X, 1x50mlDiluent 5X, 1x50mlDetection Antibody, 1x12mlDetector 100X (HRP Labeled Streptavidin), 1x150ulMouse ANGPTL3 Standard, lyophilized, 1x2ngMouse ANGPTL3 QC sample, lyophilized, 1x vialTMB Substrate Solution, 1x12mlStop Solution, 1x12mlPlate sealers, 3x plastic filmStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對ANGPTL3, BioAssay ELISA Kit (Mouse, Rat) (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到ANGPTL3, BioAssay ELISA Kit (Mouse, Rat) (Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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