- 中文名稱
IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)
- 英文名字
- IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)
- 供應商
- USBiological
- 產品貨號
- 207234
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not re- quired for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent mannerSample Type:Cell culture supernatants, serum and plasmaIntended Use:The mouse IL-1 alpha BioAssay ELISA Kit is to be used for the in vitro quantitative determination of mouse IL-1alpha in cell culture supernatants, serum and plasma. Sensitivity:9pg/mlRange:15.6pg/ml-1ng/mlSpecificity:Recognizes natural and recombinant mouse IL-1alpha (proform and p17 fragment). Does not detect human IL-1alpha or mouse IL-1beta.Test Principle:This BioAssay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse IL-1alpha in cell culture supernatants, serum and plasma. A monoclonal antibody specific for IL-1alpha has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-1alpha is recognized by the addition of a biotinylated monoclonal antibody specific for IL-1alpha (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of IL-1alpha in the samples.Kit Components:Mouse IL-1alpha Standard, lyophilized, 1x1ugDetection Antibody, 1x20ulHRP Labeled Streptavidin, lyophilized, 1x2ugWash Buffer 10X, 2x30mlELISA Buffer 10X, 2x30mlTMB Substrate Solution, 1x12mlStop Solution, 1x12mlPlate coated with mIL-1alpha antibody, 6x16 well stripsPlate covers, 2x plastic filmSilica gel, 2x minibagsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到IL-1alpha, Mouse, BioAssay ELISA Kit (Interleukin-1alpha IL-1F1)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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