- 中文名稱
Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)
- 英文名字
- Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)
- 供應商
- USBiological
- 產品貨號
- 215993
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
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- 背景資料
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- 產品描述
- Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is a member of the hematopoietic cytokine family, which includes interleukin-3 (IL-3) and interleukin-5 (IL-5).?The non-glycosylated recombinant mouse GM-CSF has a calculated molecular weight of 14kD.?Because of the formation of intramolelular disulfide bonds and glycosylation, GM-CSF migrates at different sizes on SDSPAGE gel.?Human GM-CSF shows 56-60% amino acid homology to murine GM-CSF but does not exhibit cross-species biological activity or receptor binding. ?GM-CSF is a potent stimulator to the marrow granulocyte/monocyte progenitor cells and promotes the function of mature granulocytes and monocytes. GM-CSF is synthesized upon stimulation by inflammatory agent or antigen. GM-CSF can be produced by and act upon a variety cell types, including T-lymphocytes, monocytes/macrophages, B-lymphocytes, endothelial cells, fibroblasts, stromal cells, mesothelial cells, keratinocytes, osteoblasts, uterine epithelial cells, synoviocytes, mast cells and various solid tumors.?Mouse GM-CSF receptor is comprised of a unique alpha subunit and a common beta subunit which is shared by IL-3 and IL-5 receptor.?GM-CSF receptor is abundant on progenitor and mature granulocytes and monocytes. The low level expression of GM-CSF receptor on non-hematopoietic cells may explain the phenomenon that higher GM-CSF concentration is required for producing biological effects on these cells.?GM-CSF stimulates macrophage production of TNF, M-CSF, G-CSF, and IL-1, and promotes the killing of infectious agents by granulocytes and macrophages.?Increased GM-CSF levels were found to be associated with various inflammatory and pathological conditions including pneumonia, asthma, psoriasis, pulmonary fibrosis, rheumatoid arthritis, systemic lupus erythematosus, myelodysplastic syndrome (MDS), thrombocytopenia, lung cancer, acute mylogenous leukemia, and tumor related thrombocytosis, GM-CSF showed therapeutic value in accelerating neutrophil recovery at myelo-suppression conditions, such as bone marrow transplantation, chemotherapy, and infectious disease.This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.Intended Use:This Mouse GM-CSF ELISA Kit is to be used for the in vitro quantitative determination of mouse granulocyte macrophage colony stimulating factor (GM-CSF) concentrations in serum, plasma, cell culture supernatant, and other biological fluids.Sensitivity:<17pg/mlRange:50-3200pg/mlTest Principle:This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to “sandwich” the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3’5,5’ tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.In order to measure the concentration of mouse GM-CSF in the samples, this kit includes two diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold dilution series) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus mouse GM-CSF concentration (pg/ml). The concentration of mouse GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This mouse GM-CSF ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse GM-CSF in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It showed no cross reactivity with other cytokines tested.Kit Components:MOUSE GM-CSF MICROTITER PLATEBIOTIN CONJUGATEAVIDIN CONJUGATEMOUSE GM-CSF STANDARD CALIBRATOR DILUENT ICALIBRATOR DILUENT IIWASH BUFFER (20X/96 wells, 30X/192 wells) SUBSTRATE A SUBSTRATE B STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Granulocyte Macrophage Colony Stimulating Factor, Mouse, BioAssay ELISA Kit (GM-CSF)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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