- 中文名稱
Interleukin-13, Human, BioAssay ELISA Kit (IL-13)
- 英文名字
- Interleukin-13, Human, BioAssay ELISA Kit (IL-13)
- 供應商
- USBiological
- 產品貨號
- 216007
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- IL-13 is a 10kD unglycosylated protein, secreted mainly by activated T helper 2 cells.? IL-13 binds to IL-4ra in addition to IL-13Ra1 and IL-13Ra2. And the function of IL-13 partially overlaps with IL-4.? Both IL-4 and IL-13 link to signal transducer and activator of transcription 6 (STAT6) through the receptor activation.?IL-13 can induce B-cell proliferation and IgE classs switching. However, IL-13 is less competent in this function comparing with IL-4. Unlike IL-4, IL-13 does not play an important role in hematopoietic cell shift, such as na?ve T helper 0 differentiation to T helper 2. ??IL-I3 signaling is significant in inducing physiological changes to non-immuno cells in parasitized organs.?For example, IL-13 induces enhanced gut contractions and glycoprotein hypersecretion which lead to the detachment of helminthes from gut wall.?Eggs produced by Schistosoma mansoni can be limited inside granulomas formed through IL-13 induced cell changes.?To organisms resided inside cells, IL-13 seemed to inhibit the clearance from host cell through antagonizing Th1 responses.IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells.? All of them contribute to airway obstruction.?Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.?Intended Use:This Human Interleukin 13 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 concentrations in cell culture supernatant. Sensitivity:<2pg/mlRange:15.6-1000pg/mlTest Principle:This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.In order to measure the concentration of IL-13 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 concentration. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:L-13?MICROTITER PLATE?BIOTIN CONJUGATE?AVIDIN CONJUGATE?IL-13?STANDARD??CALIBRATOR DILUENT I?CALIBRATOR DILUENT II?WASH BUFFER (20X/96 wells, 30X/192 wells)??SUBSTRATE A??SUBSTRATE B??STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Interleukin-13, Human, BioAssay ELISA Kit (IL-13)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Interleukin-13, Human, BioAssay ELISA Kit (IL-13)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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