- 中文名稱
Interleukin-2, Mouse, BioAssay ELISA Kit (IL-2)
- 英文名字
- Interleukin-2, Mouse, BioAssay ELISA Kit (IL-2)
- 供應商
- USBiological
- 產品貨號
- 216012
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- This mouse IL-2 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay.?The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-2. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse IL-2, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-2 and other components in the samples. A biotin-conjugated antibody preparation specific for mouse IL-2 is added to each well and incubated.?The biotin labelled antibody will attach to the wells by binding to mouse IL-2 present in the standards/samples.?After plate washing, other proteins, components and unattached biotin labelled antibody are removed.?Then, avidin-horseradish peroxidase (HRP) conjugate is added to each well.?Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin conjugated antibody. The wells are thoroughly washed to remove all unbound avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only the wells that contain mouse IL-2 will exhibit a change in color.?The extent of color change is proportional to the quantity of mouse IL-2 present in the standards/samples.?The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of mouse IL-2 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing).?According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples.?This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-2 concentration (pg/ml). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Intended Use:This Mouse IL-2 ELISA kit is to be used for the in vitro quantitative determination of mouse interleukin 2 (IL-2) concentrations in serum, cell culture supernatant, and other biological fluids.Sensitivity:<2.1pg/mlRange:7.8-500pg/mlTest Principle:This mouse IL-2 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-2. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse IL-2, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-2 and other components in the samples. A biotin-conjugated antibody preparation specific for mouse IL-2 is added to each well and incubated. The biotin labelled antibody will attach to the wells by binding to mouse IL-2 present in the standards/samples. After plate washing, other proteins, components and unattached biotin labelled antibody are removed. Then, avidin-horseradish peroxidase (HRP) conjugate is added to each well. Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin conjugated antibody. The wells are thoroughly washed to remove all unbound avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only the wells that contain mouse IL-2 will exhibit a change in color. The extent of color change is proportional to the quantity of mouse IL-2 present in the standards/samples. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of mouse IL-2 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-2 concentration (pg/ml). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:MOUSE IL-2 MICROTITER PLATE?BIOTIN CONJUGATE?AVIDIN CONJUGATE?MOUSE IL-2 STANDARD??CALIBRATOR DILUENT I?CALIBRATOR DILUENT II?WASH BUFFER (20X/96 wells, 30X/192 wells)??TMB SUBSTRATE??STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Interleukin-2, Mouse, BioAssay ELISA Kit (IL-2)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Interleukin-2, Mouse, BioAssay ELISA Kit (IL-2)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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