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中文名稱

Fumarase Activity Colorimetric BioAssay Kit (Fumarate Hydratase, EC 4.2.1.2)

英文名字
Fumarase Activity Colorimetric BioAssay Kit (Fumarate Hydratase, EC 4.2.1.2)
供應商
USBiological
產品貨號
219778
產品報價
¥1/100Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Fumarase (Fumarate Hydratase: EC 4.2.1.2) is an enzyme that catalyzes the reversible reaction of hydration and dehydration of fumarate to Malate. It has two forms: mitochondrial and cytosolic forms. The mitochondrial form of fumarase is one of the key enzymes in citric acid cycle, while cytosolic form is important for metabolism of amino acids and fumarate. In humans, fumarase deficiency leads to serious health problems such as fetal brain abnormality, hypotonia, and renal cell carcinoma. Therefore, accurate measurement of fumarase activity is important for preventing, diagnosis and mechanistic study of fumarase deficiency. Sample Type:Animal tissues: liver, heart, muscle etc. Cell culture: Adherent or suspension cells. Isolated mitochondria.?Intended Use:For use in the measurement of fumarase activity in various tissues/cells and analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.Sensitivity:This kit can detect <50U/ml of fumarase activity in variety of sample types.Test Principle:The Fumarase Activity BioAssay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450nm. Kit Components:Fumarase Assay Buffer, 1x22ml ?Fumarase Substrate, 1x0.2ml ?Fumarase Enzyme Mix, 1x vial ?Fumarase Developer, 1x vial ?NADH Standard, 1x vial ?Fumarase Positive Control, 1x vialStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Fumarase Activity Colorimetric BioAssay Kit (Fumarate Hydratase, EC 4.2.1.2) 該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Fumarase Activity Colorimetric BioAssay Kit (Fumarate Hydratase, EC 4.2.1.2) 產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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