- 中文名稱
Lipolysis, 3T3-L1, Colorimetric BioAssay Kit
- 英文名字
- Lipolysis, 3T3-L1, Colorimetric BioAssay Kit
- 供應商
- USBiological
- 產品貨號
- 220010
- 產品報價
- ￥1/100Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamines and certain hormones.Sample Type:Primary adipocytes. Cell culture: 3T3-L1 cells.Intended Use:Measurement of lipolysis in 3T3-L1 cells or adipocytes. Screening compounds that influence lipolysis, mechanistic studies and studies on metabolic dysfunctions.Sensitivity:This assay kit can detect <200pmol of glycerol.Test Principle:This kit includes synthetic catecholamines, Isoproterenol, which activates beta-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone- sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. This 3T3-L1 Lipolysis BioAssay kit is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. Kit Components:Lipolysis Assay Buffer, 1x17ml Lipolysis Wash Buffer, 1x22mlGlycerol Assay Buffer, 1x25mlGlycerol Probe, in DMSO, Anhydrous, 1x0.2ml Glycerol Enzyme Mix, lyophilized, 1x vial Glycerol Standard, 100mM, 1x0.2ml Isoproterenol, 10mM, 1x50ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Lipolysis, 3T3-L1, Colorimetric BioAssay Kit 該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Lipolysis, 3T3-L1, Colorimetric BioAssay Kit 產品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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