- 中文名稱
Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)
- 英文名字
- Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)
- 供應商
- USBiological
- 產品貨號
- 220212
- 產品報價
- ￥1/100Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress.Sample Type:Animal tissues: heart, liver, muscle, etc. Cell culture: Adherent or suspension cells. Purified mitochondria.Intended Use:For use in the measurement of pyruvate dehydrogenase activity in various tissues/cells and for analysis of the cell signaling pathway.Sensitivity:Can measure pyruvate dehydrogenase activity lower than 0.1mU in a variety of samples.Test Principle:This PDH BioAssay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450nm. Kit Components:PDH Assay Buffer, 1x25mlPDH Substrate, lyophilized, 1x vial PDH Developer, lyophilized, 1x vial NADH Standard, lyophilized, 1x vial PDH Positive Control, 1x10ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Pyruvate Dehydrogenase Activity Colorimetric BioAssay Kit (PDH)產品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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