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中文名稱

Thioredoxin Reductase Activity, BioAssay Kit, Colorimetric (TrxR)

英文名字
Thioredoxin Reductase Activity, BioAssay Kit, Colorimetric (TrxR)
供應商
USBiological
產品貨號
220324
產品報價
¥1/100Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.Sample Type:Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc. Specificity:Recognizes mammalian TrxR.Test Principle:The Thioredoxin Reductase BioAssay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (lmax = 412nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.Kit Components:TrxR Assay Buffer, 1x25ml?TNB Standard, lyophilized, 1x vial?DTNB, lyophilized, 1x vial?NADPH, lyophilized, 1x vial?TrxR Positive Control, 1x vial?TrxR Inhibitor, lyophilized, 1x vialStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Thioredoxin Reductase Activity, BioAssay Kit, Colorimetric (TrxR)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Thioredoxin Reductase Activity, BioAssay Kit, Colorimetric (TrxR)產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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