- 中文名稱
ACES Signal Sequence BioAssay Kit
- 英文名字
- ACES Signal Sequence BioAssay Kit
- 供應商
- USBiological
- 產品貨號
- 226803
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- E. coli is often the host strain of first choice for the production of recombinant proteins. Despite its long and successful history, several limitations remain when the desired protein cannot be expressed in a functional state. This is often due to the inherent properties of expressingheterologous proteins in the cytoplasm. One means of overcoming these impediments is to express the protein such that it is secreted into an environment that is more conducive to correct folding and where proteolytic degradation is minimized, such as the periplasm or extracellular matrix. Periplasmic or extracellular protein export has been exploited for the production of a number of recombinant proteins. The extracellular accumulation of a target protein provides several advantages. These include: A Simplified Downstream Purification Scheme, Enhanced Biological Activity, Higher Product Stability and Solubility.Protein secretion in E. coli and other Gram-negative bacteria is an extensively reviewed subject.1 It is also a very complex process. The pAES30 series vectors utilize signal sequences that represent the three translocation pathways of the type II secretion process of E. coli.The type II secretion mechanism is a two-step process. Protein translocation through the inner membrane is accomplished by way of one of three pathways: SecB-dependent (SEC), signal recognition particle (SRP) or twin-arginine translocation (TAT) (Fig. 1). Most proteins in E. coli are exported to the periplasm via one of these pathways with the larger majority using SEC. Extracellular secretion by a type II mechanism is via the main terminal branch pathway, which involves a poorly defi ned complex of 12-16 proteins that are not expressed under normal laboratory conditions.2 Therefore, most of the protein exported by the type II systems remains resident in the periplasm, which is also where most secreted recombinant proteins accumulate.3Test Principle:Six signal sequences representing the three translocation pathways of E. coli (SRP, SEC, TAT) are fused to the N-terminus of the Target Protein facilitating its secretion to the periplasmic space. Translocation through the inner membrane causes removal of the signal sequence resulting in a Target Protein with its native N-terminus. Once in the periplasmic space, the Target Protein can then be recovered by using any number of reagents that perforate the outer cell membrane, causing release of the Target Protein into the culture medium. Alternatively, cells can be harvested by centrifugation and a cold osmotic shock protocol used to extract the recombinant Target Protein from the periplasmic space.Kit Components:pAES30, 1x10ugpAES31, 1x10ugpAES32, 1x10ugpAES33, 1x10ugpAES34, 1x10ugpAES35, 1x10ugJM109 Competent Cells, 2x200ul LB Broth (Miller) 1x1L MixTurbo Broth 1x1L MixSuperior Broth 1x1L MixPower Broth 1x1L MixHyper Broth 1x1L MixGlucose M9Y 1x1L MixGlucose Nutrient Mix, 1x21gAugmedium 25ml mixInducer Solution A, 1x 1mlInducer Solution B, 1x100mlSecretion Enhancer Solution A 1x60mlSecretion Enhancer Solution B, 1x60mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對ACES Signal Sequence BioAssay Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到ACES Signal Sequence BioAssay Kit產品后將其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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