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中文名稱

Adiponectin BioAssay ELISA Kit (ACRP30, AdipoQ, apM1, GBP28, Adipocyte complement related protein of 30kD) (Human)

英文名字
Adiponectin BioAssay ELISA Kit (ACRP30, AdipoQ, apM1, GBP28, Adipocyte complement related protein of 30kD) (Human)
供應商
USBiological
產品貨號
226805
產品報價
¥1/96wells
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Adiponectin [ACRP30; AdipoQ] is a promising biomarker of insulin resistance and type 2 diabetes mellitus (T2DM) but also as a potential target for management of the metabolic syndrome. It is a very robust marker that is not prone to degradation or acute inflammatory challenges, is present in relatively high concentrations in the peripheral circulation, and can be collected by a variety of methods. The benefits of using adiponectin assays in clinical settings include, (a) prediction of risk of diabetes and metabolic status and (b) providing a tool to monitor metabolic improvements. Adiponectin exerts anti-atherogenic and anti-inflammatory properties and may be important as a biomarker for obesity-related cardiovascular disease (CVD). New findings showed urinary adiponectin excretion as an independent new biomarker of microvascular and macrovascular damage in T2DM and suggested it as a very promising tool for early cardiovascular disease risk assessment. Adiponectin serum level was also described as a good biomarker of colorectal adenoma, this being related to the positive correlation between obesity and increased risk of cancer at various sites (colorectal, breast, prostate and endometrium). Sample Type:Cell Culture Supernatant, Plasma, Serum, Urine Intended Use:The Adiponectin BioAssay ELISA Kit is to be used for the in vitro quantitative determination of human Adiponectin in serum, plasma, urine and cell culture supernatant. This ELISA Kit is for research use only.Sensitivity:100pgRange:0.5-32ng/ml Specificity:Detects human adiponectin. Does not cross-react with mouse adiponectin, rat adiponectin, human resistin, human RELM-beta or human leptin. Test Principle:This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human adiponectin in biological fluids. A monoclonal antibody specific for adiponectin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, adiponectin is recognized by the addition of a purified polyclonal antibody specific for adiponectin (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of adiponectin in the samples.Kit Components:Coated plate, 1x1Wash Buffer 10X, 2x30mlELISA Buffer 10X, 2x30mlDetection Antibody, 1x30ulHRP 100X (HRP Conjugated anti-rabbit IgG), 1x150ulAdiponectin Standard (lyophilized), 1x64ng TMB Substrate Solution, 1x12mlStop Solution, 1x12ml2 plate sealers (plastic film) 2 silica Gel MinibagsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Adiponectin BioAssay ELISA Kit (ACRP30, AdipoQ, apM1, GBP28, Adipocyte complement related protein of 30kD) (Human)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Adiponectin BioAssay ELISA Kit (ACRP30, AdipoQ, apM1, GBP28, Adipocyte complement related protein of 30kD) (Human)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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