- 中文名稱
Equine IgG (Immunoglobulin G) BioAssay ELISA Kit
- 英文名字
- Equine IgG (Immunoglobulin G) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- 227732
- 產品報價
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Equine IgG (Immunoglobulin G) BioAssay ELISA Kit is a competitive ELISA intended for the quantitative determination of IgG concentration in equine serum and plasma.Detection Range:25-400ug/ml Sensitivity:<23.764ug/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with equine IgG. Standards, samples and HRP-conjugated anti-equine IgG are added to the appropriate microtiter plate wells and a competitive reaction is launched between plate-coated equine IgG and equine IgG in the standards or samples for limited antibody binding sites on the HRP-conjugated anti-equine IgG reagent. The wells are washed to remove unbound materials. TMB substrate solution is then added to the wells; color development is stopped and absorbance read at 450nm wavelength. The intensity of the color is inversely proportional to the amount of equine IgG in the samples.Kit Components:227665A: Microtiter Strips, 1x96 wells. Pre-coated; ready to use. 227665B: Standard, 0ug/ml, 1x500ul227665C: Standard, 25ug/ml, 1x500ul227665D: Standard, 50ug/ml, 1x500ul227665E: Standard, 100ug/ml, 1x500ul227665F: Standard, 200ug/ml, 1x500ul227665G: Standard, 400ug/ml, 1x500ul227665H: HRP Conjugate, 1x6ml227665J: Sample Diluent 2x20ml227665K: Wash Buffer, 25X, 1x20ml227665L: TMB Substrate, 1x10ml227665M: Stop Solution, 1x10mlNote: The Stop Solution (227732K) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents and samples as instructed.2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well.4. Add 50ul HRP Conjugate to each well (do not add any to the blank well).5. Incubate for 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate to each well.8. Incubate for 20 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 產品特點
- 針對Equine IgG (Immunoglobulin G) BioAssay ELISA Kit 該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Equine IgG (Immunoglobulin G) BioAssay ELISA Kit 產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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