- 中文名稱
Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)
- 英文名字
- Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 227814
- 產品報價
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.Intended Use:The Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human) BioAssay ELISA Kit is a sandwich ELISA intended for the quantitative determination of MCP-1 concentration in human serum, plasma, tissue homogenate, cerebrospinal fluid, saliva, urine, ascitic fluid and cell culture supernatant. Sensitivity:<30.184pg/mlDetection Range:31.25-2000pg/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of human MCP-1. No significant cross-reactivity or interference between human MCP-1 and analogues was observed.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Principle of the Assay:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-1 is added to the wells. After washing, avidin-conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:227814A: Microtiter Strips, 12x8 Microwell Strips. Pre-coated; ready to use. 227814B: Standard (lyophilized), 2 vials227814C: Biotin-Antibody (100x Concentrate), 1x120ul227814D: HRP-Avidin, (100x Concentrate), 1x120ul227814E: Biotin-Antibody Diluent, 1x15ml227814F: HRP-Avidin Diluent, 1x15ml227814G: Sample Diluent, 1x50ml227814H: Wash Buffer (25X Concentrate), 1x20ml227814J: TMB Substrate, 1x10ml227814K: Stop Solution, 1x10mlStorage and Stability:Store all kit components at 4°C. Unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for 1 month provided this is within the expiration date of the kit.Opened Kit: Coated plate may be stored at 4°C for up to 1 month, sealed in the aluminum foil bag. Standard, Biotin-Antibody and HRP-Avidin may be stored at 4°C or -20°C for up to 1 month. The rest of the components may be stored at 4°C for up to 1 month.For maximum recovery of contents, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, standards and samples as instructed.2. Add 100ul standard or sample to each well. Incubate for 2 hours at 37°C3. Remove the liquid of each wee; do not wash.4. Add 100ul Biotin-Antibody (1x) to each well. Incubate for 1 hour at 37°C5. Aspirate and wash 3 times.6. Add 100ul HRP-Avidin (1x) to each well. Incubate for 1 hour at 37°C7. Aspirate and wash 5 times.8. Add 90ul TMB substrate to each well Incubate for 15-30 minutes at 37°C. Protect from light9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
- 產品特點
- 針對Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Monocyte Chemotactic Protein 1 (MCP-1) BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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