- 中文名稱
SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)
- 英文名字
- SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)
- 供應商
- USBiological
- 產品貨號
- 298456
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- SETD2 is a histone methyltransferase that exhibits methylation activity toward histones H3-K36.Intended Use:The SETD2 Direct Activity Chemiluminescent BioAssay Kit is designed to measure SETD2 activity for screening and profiling applications. Test Principle:The key to the SETD2 Chemiluminescent BioAssay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Contraindications:Avoid green and blue dyes that absorb light in the Alphascreen? signal emission range (520-620nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen? assays.Applications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Plate precoated with histone substrate, 1x96 wellsSETD2 human enzyme, 1x40ug S-adenosylmethionine, 1x250ul Primary antibody 12, 1x100ul Secondary HRP-labeled antibody 2, 1x10ul 4x HMT assay buffer 1, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate, 2 components x 6ml each Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)產品后將其置于4°C/-20°C/-70°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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