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細胞分析試劑盒
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中文名稱

DNA Isolation Kit, Saliva, BioAssay

英文名字
DNA Isolation Kit, Saliva, BioAssay
供應商
USBiological
產品貨號
299009
產品報價
¥1/50Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Saliva or septum samples, the isolation procedure was designed for purification from 200ul of sample but may be used to isolate DNA from up to 600ul of saliva sample. If increasing the sample volume from 200ul, adjust the amount of Lysis Binding Buffer C, Proteinase K Solution, and Isopropanol proportionately.Intended Use:The Saliva DNA Isolation BioAssay Kit is designed for the purification of genomic DNA and circulating DNA from freshly collected saliva samples. Range:50-200ul of sampleDNA Yield: 0.25-4ug/200ul fresh salivaTest Principle:Purification is performed with the use of Spin columns which bind the DNA. No phenol-chloroform and precipitation steps are involved in this procedure. Isolation of the DNA is performed by lysing the cells in a solution containing chaotropic salts and detergent. To assist inlysing the cells, proteinase K is also added to the mixture. Isopropanol is added to the samples, before applying to the spin columns. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.Kit Components:Lysis Binding Buffer C, 1x30ml, RTWash Buffer 1, 1x14ml, RTWash Buffer 2, 1x7ml, RTTE Buffer, 1x10ml, RTSpin column set, 50 units, RTProteinase K Solution, 1x250ul, -20°C* Wash buffers 1 and 2 are provided as concentrates that require the addition of 100% ethanol to them before use.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對DNA Isolation Kit, Saliva, BioAssay 該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到DNA Isolation Kit, Saliva, BioAssay 產品后將其置于RT/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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