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中文名稱

Optimax First Strand cDNA Synthesis BioGenomics Kit

英文名字
Optimax First Strand cDNA Synthesis BioGenomics Kit
供應商
USBiological
產品貨號
299025
產品報價
¥1/100Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
First Strand cDNA Synthesis BioGenomics Kit is developed based on cDNA synthesis protocol. The kit generates full-length first strand cDNA that is ready for PCR amplification. An easy to follow protocol is provided. The kit contains sufficient reagents for 100 cDNA synthesis reactions.Intended Use:Immediate PCR amplification of known genesComparison of gene expression patterns between biological samplesGene mutation analysisGene cloning and target sequencingTest Principle:This First Strand cDNA Synthesis BioGenomics Kit provides all necessary components to perform 100 first strand cDNA synthesis reactions from total RNA or poly(A) RNA. The system uses Moloney Murine Leukemia Virus Reverse Transcriptase with weak activity of RNase H that allows the synthesis of full-length cDNA from long templates. Oligo(dT)18 and random hexamer primers are provided. Specific primers can also be used with the kit.The cDNA synthesized using this system can be used directly in PCR amplification. The kit is optimized for maximum yield of full-length cDNA. The addition of ribonuclease inhibitor lowers the risk of mRNA degradation during the reaction.Quality Control:All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy in first strand cDNA synthesis.Kit Components:MMLV reverse transcriptase, 100unit/ul, 1x100ulReaction Buffer, 5x, 1x1mlOligo(dT)18, 0.5ug/ul, 1x100ulRandom Hexamers, 0.2ug/ul, 1x100ulRNasein, 10units/ul, 1x100uldNTP, 10mM each, 1x250ulHuman Placenta total RNA, 1.25ug/ul, 1x10ulUpstream Primer, 25um, 1x50ulDownstream Primer, 25um, 1x50ulDEPC H2O, 1x1.25mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Optimax First Strand cDNA Synthesis BioGenomics Kit該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Optimax First Strand cDNA Synthesis BioGenomics Kit產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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