PARP，a 116 kDa nuclear poly （ADP-ribose） polymerase，is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215，which separates PARP’s N-terminal DNA binding domain （24 kDa） from its C-terminal catalytic domain （89 kDa）. It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
應用類型
Immunocytochemistry : 5-20 ug/ml； Western Blot （10 ug/ml using colorimetric methods； <2 ug/ml for ECL）； Immunoprecipitation and ELISA
免疫原
Poly（ADP-ribose） mixed with methylated bovine serum albumin
來源宿主
小鼠
反應性
經檢測驗證，可與哺乳動物種屬來源的PARP蛋白發生免疫反應
保存建議
提供的100 ug PARP抗體（克隆號10H）產品為無色溶液其濃度為1.0 mg/ml溶解于包含有1% BSA、0.02%疊氮化鈉和150 mM NaCl的20 mM Tris （pH 7.4）緩沖液中.請將該PARP抗體（克隆號10H）產品置于-20 °C冷凍保存，建議分裝使用以避免反復凍融.
