Fas-associating death domain containing protein （FADD），also known as mediator of receptor induced toxicity （MORT1），is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas，an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA，a poxvirus protein that blocks both Fas- and TNF-induced cell death.
應用類型
Western blot analysis （1-4 ug/ml）. The antibody detects 28 kDa protein， corresponding to the apparent molecular weight of FADD in SDS-PAGE However， the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.immunoblots from samples of human and mouse origins.
免疫原
Recombinant human FADD
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、狗種屬來源的FADD蛋白 發生免疫反應.
保存建議
提供的FADD抗體 產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該FADD抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
