The caspase family of cysteine proteases plays a key role in apoptosis. Caspase-7，also known as Mch3，ICE/LAP3 and CMH1，is a 34 kDa protein that is activated during Fas- and TNF-induced apoptosis. Caspase-7 has been identified as a major contributor to the execution phase of cellular apoptosis. It cleaves essential cellular machinery following its own cleavage from an inactive proenzyme to an active heterodimeric form. Caspase-7 is processed by caspase-3，-6，-8，-9，and –10 as well as by Granzyme B.
應用類型
Western blot analysis （0.5-2 ug/ml） and immunoprecipitation （4-10 ug/ml）. However the optimal conditions should be determined individually. Detects both pro-caspase-7 （34 kDa） and the large subunit of cleaved caspase-7 （19 kDa） in apoptosis cells on SDS-PAGE immunoblots.
免疫原
Synthetic peptide from human caspase-7.
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Caspase-7蛋白發生免疫反應.
保存建議
提供的Caspase-7抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM硫柳汞和50%甘油的PBS緩沖液中.請將該Caspase-7抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
