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中文名稱

Apolipoprotein E, BioAssay ELISA Kit (Human) (APOE)

英文名字
Apolipoprotein E, BioAssay ELISA Kit (Human) (APOE)
供應商
USBiological
產品貨號
301179
產品報價
¥1/100Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
This Human Apolipoprotein E (ApoE) Enzyme-Linked Immunosorbent Assay (ELISA) BioAssay Kit is an in vitro assay for the quantitative measurement of human ApoE. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. It is synthesized mainly in liver, while it has also been identified in the brain, kidneys, and spleen. In the nervous system, ApoE is synthesized in non-neuronal cell types, most notably astroglia and microglia, while neurons preferentially express its receptors. Defects in ApoE lead to familial dysbetalipoproteinemia (increased plasma cholesterol and triglycerides). More recently, ApoE has been implicated in several biological processes not directly related to lipoprotein transport, including Alzheimers disease, immunoregulation, and cognition. Sample Type:Serum, plasma, cell lysate, cell culture supernatant, and cerebrospinal fluid (CSF)Intended Use:Quantitative measurement of human Apolipoprotein E (ApoE).Sensitivity:25ng/mlRange:25ng-1.6ug/mlSpecificity:This ELISA kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms. Species Crossreactivity: humanTest Principle:This BioAssay employs an antibody specific for human ApoE coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. Recovery inside this range is between 86 and 111% (average recovery is 102%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is <8% & inter-assay has CV <12%.Kit Components:Plate Coated with ApoE Ab, 1x96 wells Assay Diluent, 1x27ml Wash Buffer A, 10x, 1x10ml Wash Buffer B, 10x, 1x20ml rh ApoE Standard, lyohilized, 3x1vial Detection Antibody, 100X, 1x110ul HRP Conjugate, 100X, 1x135ul TMB Substrate, 1x11ml Stop Solution, 1x11ml Plate Sealer, 2x sealers Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對Apolipoprotein E, BioAssay ELISA Kit (Human) (APOE)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Apolipoprotein E, BioAssay ELISA Kit (Human) (APOE)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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