NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit （p50） and a 65 kDa transactivation subunit （p65/RelA）. Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor，IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide （LPS），pro-inflammatory cytokines，and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
應用類型
Western blot analysis （1-4 ug/ml）. However， the optimal conditions should be determined individually. A431 cells can be used as a positive control.
免疫原
Synthetic peptide from human NFkB
來源宿主
小鼠
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的NFkB p65蛋白發生免疫反應.
保存建議
提供的NFkB p65抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 疊氮鈉和50%甘油.的PBS緩沖液中.請將該NFkB p65抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
